Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue
Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue
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DOI：
10.1017/s0007114511004934
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发表时间：
2012-06-01
影响因子：
3.6
通讯作者：
Lin, Tzou-Yien
中科院分区：
文献类型：
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作者：
Chen, Chien-Chang;Lin, Wei-Chuan;Lin, Tzou-Yien
通过给予益生菌调节细胞反应可能是预防或抑制肿瘤生长的有效策略。将益生菌Lactobacillus acidophilus NCFM（La）经口预接种小鼠14 d。使用CT-26鼠结肠腺癌细胞将皮下背侧肿瘤和节段性原位结肠癌植入小鼠体内。在肿瘤开始后第28天，收获结肠、肠系膜淋巴结（MLN）和脾的固有层并纯化用于流式细胞术和mRNA分析。我们证明，在肿瘤植入后28天，与未处理的小鼠相比，La预接种使肿瘤体积生长减少了50.3%（2465.5（SEM 1290.4）v. 4950.9（SEM 1689.3）mm（3），P
Modulation of the cellular response by the administration of probiotic bacteria may be an effective strategy for preventing or inhibiting tumour growth. We orally pre-inoculated mice with probiotics Lactobacillus acidophilus NCFM (La) for 14 d. Subcutaneous dorsal-flank tumours and segmental orthotopic colon cancers were implanted into mice using CT-26 murine colon adenocarcinoma cells. On day 28 after tumour initiation, the lamina propria of the colon, mesenteric lymph nodes (MLN) and spleen were harvested and purified for flow cytometry and mRNA analyses. We demonstrated that La pre-inoculation reduced tumour volume growth by 50.3%, compared with untreated mice at 28 d after tumour implants (2465.5 (SEM 1290.4) v. 4950.9 (SEM 1689.3) mm(3), P