Cortical microtubule contacts position the spindle in C. elegans embryos
Cortical microtubule contacts position the spindle in C. elegans embryos
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DOI：
10.1016/j.cell.2007.03.027
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发表时间：
2007-05-04
期刊：
影响因子：
64.5
通讯作者：
Nedelec, Francois
中科院分区：
文献类型：
--
作者：
Kozlowski, Cleopatra;Srayko, Martin;Nedelec, Francois
微管和细胞皮层之间的相互作用在各种生物学环境中定位细胞器中起着关键作用。在这里，我们使用秀丽隐杆线虫作为一个模型系统，研究如何皮质微管相互作用的位置有丝分裂纺锤体的极性线索。EBP-2：：GFP和YFP：：octubulin的成像显示，微管在皮质接触后很快收缩，由此我们提出皮质适配器介导微管解聚能量转化为拉力。我们还观察到动态微管的关联，以形成持续存在的星形纤维，尽管个别微管的灾难事件。计算机模拟表明，这些影响，这是至关重要的微管动力学决定，可以解释后期纺锤体振荡和三维后位移。
Interactions between microtubules and the cell cortex play a critical role in positioning organelles in a variety of biological contexts. Here we used Caenorhabditis elegans as a model system to study how cortex-microtubule interactions position the mitotic spindle in response to polarity cues. Imaging EBP-2::GFP and YFP::octubulin revealed that microtubules shrink soon after cortical contact, from which we propose that cortical adaptors mediate microtubule depolymerization energy into pulling forces. We also observe association of dynamic microtubules to form astral fibers that persist, despite the catastrophe events of individual microtubules. Computer simulations show that these effects, which are crucially determined by microtubule dynamics, can explain anaphase spindle oscillations and posterior displacement in 3D.