Anti-DNA antibody in procainamide-induced lupus erythematosus. Determinations using DNA fractionated by methylated albumin-Kieselguhr chromatography.
Anti-DNA antibody in procainamide-induced lupus erythematosus. Determinations using DNA fractionated by methylated albumin-Kieselguhr chromatography.
复制标题
普鲁卡因酰胺诱导的红斑狼疮中的抗 DNA 抗体。
作者：
John B. Winfield;John S. Davis
通过甲基化白蛋白-硅藻土层析法测定，纯化的天然（n）市售小牛胸腺DNA和14 C标记的人KB DNA均含有大量变性DNA（dDNA）。当未分级DNA用于抗DNA抗体的反式免疫电泳或Farr试验时，这些制剂中dDNA的比例足以引起抗DNA抗体抗原特异性的混淆。系统性红斑狼疮患者血清中同时含有抗nDNA和抗dDNA抗体。大约50%的有症状的普鲁卡因胺诱导的狼疮（PLE）患者表现出抗dDNA;没有抗nDNA。普鲁卡因胺治疗的ANF阳性患者没有症状性PLE，既没有抗dDNA也没有抗nDNA。
Both putatively native(n) commercial calf thymus DNA and 14C labeled human KB DNA contained significant amounts of denatured DNA (dDNA) as determined by methylated albumin-kieselguhr chromatography. The proportion of dDNA in these preparations was sufficient to cause confusion regarding the antigenic specificity of anti-DNA antibody when unfractionated DNA was used in either the counterimmunoelectrophoresis or the Farr assays for anti-DNA antibody. Serum from patients with systemic lupus erythematosus contained both anti-nDNA and anti-dDNA antibodies. About 50% of patients with symptomatic procainamide-induced lupus (PLE) exhibited anti-dDNA; none had anti-nDNA. Procainamide treated patients with a positive ANF who did not have symptomatic PLE had neither anti-dDNA nor anti-nDNA.