Capturing protein droplets: label-free visualization and detection of protein liquid-liquid phase separation with an aggregation-induced emission fluorogen
Capturing protein droplets: label-free visualization and detection of protein liquid-liquid phase separation with an aggregation-induced emission fluorogen
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DOI：
10.1039/d1cc00947h
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发表时间：
2021-04-21
影响因子：
4.9
通讯作者：
Li, Yan-Mei
中科院分区：
文献类型：
--
作者：
Liang, Chu-Qiao;Wang, Lin;Li, Yan-Mei
我们开发了一种基于聚集诱导发射（AIE）荧光团的液滴探针（DroProbe）用于蛋白液滴可视化的新方法。还开发了一种基于DroProbe寿命的蛋白质凝聚相粘度比较的简单方法。
We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of the protein condensed phase based on the lifetime of the DroProbe was also developed.