Mesoporous silica nanoparticles grafted with a light-responsive protein shell for highly cytotoxic antitumoral therapy.
Mesoporous silica nanoparticles grafted with a light-responsive protein shell for highly cytotoxic antitumoral therapy.
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DOI：
10.1039/c5tb00304k
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发表时间：
2015-07
期刊：
影响因子：
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通讯作者：
M. Martínez-Carmona;A. Baeza;M. A. Rodriguez-Milla;J. García-Castro;M. Vallet‐Regí
中科院分区：
文献类型：
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作者：
M. Martínez-Carmona;A. Baeza;M. A. Rodriguez-Milla;J. García-Castro;M. Vallet‐Regí
介绍了一种新型的光触发药物传递纳米载体，它对人肿瘤细胞具有很高的肿瘤细胞毒性。这种装置是基于介孔二氧化硅纳米颗粒，上面装饰着一个可通过光照射裂解的生物兼容蛋白质壳。组成蛋白质外壳的蛋白质(亲和素、链霉亲和素和生物素化转铁蛋白)同时充当靶向和封闭剂，避免使用多余的系统。光响应行为是由生物素化的光解交联剂共价接枝在介孔表面上提供的，介孔表面受到紫外光(366 Nm)的光解。在非常低的粒子浓度下孵育的人肿瘤细胞在很短的照射时间后进入凋亡阶段。因此，这里描述的系统可以应用于治疗暴露的肿瘤，这些肿瘤影响到皮肤、食道和胃等，并且很容易受到光线照射。
A novel phototriggered drug delivery nanocarrier, which exhibits very high tumor cytotoxicity against human tumoral cells, is presented. This device is based on mesoporous silica nanoparticles decorated with a biocompatible protein shell cleavable by light irradiation. The proteins that compose the protein shell (avidin, streptavidin and biotinylated transferrin) act as targeting and capping agents at the same time, avoiding the use of redundant systems. The light responsive behavior is provided by a biotinylated photocleavable cross-linker covalently grafted on the mesoporous surface, which suffers photocleavage by UV radiation (366 nm). Human tumoral cells incubated in the presence of a very low particle concentration enter into the apoptotic stage after a short irradiation time. Thus, the system described here could be applied to the treatment of exposed tumors that affect the skin, oesophagus, and stomach, among others, and are easily accessible for light irradiation.