Analgesic effect of milnacipran is associated with c-Fos expression in the anterior cingulate cortex in the rat neuropathic pain model
Analgesic effect of milnacipran is associated with c-Fos expression in the anterior cingulate cortex in the rat neuropathic pain model
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DOI：
10.1016/j.neures.2009.04.010
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发表时间：
2009-08-01
影响因子：
2.9
通讯作者：
Ishida, Yasushi
中科院分区：
文献类型：
--
作者：
Takeda, Ryuichiro;Watanabe, Yuko;Ishida, Yasushi
本研究的目的是观察5-羟色胺-去甲肾上腺素再摄取抑制剂米那普兰对神经病理性疼痛大鼠是否有镇痛作用。此外，本研究还观察了大鼠坐骨神经慢性压迫性疼痛(CCI)后，c-Fos在脊髓上部位和脊髓背角的表达与伤害性加工的关系。在CCI诱导的神经病变大鼠中，进行行为学测试和c-Fos免疫组织化学检测，以确定对机械刺激的戒断阈值的变化。米那普兰的抗痛觉超敏作用随观察时间的延长而逐渐增强，提示米那普兰的延迟性镇痛作用可能是通过持续给药引起的。机械伤害性刺激引起的前扣带回c-Fos表达增加可被米那普兰持续给药显著抑制，提示米那普兰可能导致前扣带回伤害性反应的功能改变。(C)2009年爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有
The objective of the present study was to examine whether milnacipran, a serotonin-noradrenaline reuptake inhibitor, has an analgesic effect in rats with neuropathic pain. In addition, the c-Fos expression was investigated in the supraspinal sites of the brain and in the spinal dorsal horn in association with the nociceptive processing in rats with neuropathic pain produced by chronic constriction injury (CCI) in the sciatic nerve. In the CCI-induced neuropathic rats, behavioral testing for determining the change in the withdrawal threshold to mechanical stimulation and immunohistochemical detection of c-Fos were both performed. The anti-allodynic effect derived from milnacipran gradually increased over the observation period, indicating that the delayed-onset analgesia might be elicited by the continuous administration of milnacipran. The increased level of c-Fos expression in the anterior cingulate cortex (ACC) induced by noxious mechanical stimulation was significantly inhibited by the continuous administration of milnacipran, indicating that milnacipran might cause a functional modification in the nociceptive processing in the ACC. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved