Effects of vitamin K2 and risedronate on bone formation and resorption, osteocyte lacunar system, and porosity in the cortical bone of glucocorticoid-treated rats
Effects of vitamin K2 and risedronate on bone formation and resorption, osteocyte lacunar system, and porosity in the cortical bone of glucocorticoid-treated rats
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DOI：
10.1007/s00223-008-9146-1
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发表时间：
2008-08-01
影响因子：
4.2
通讯作者：
Yeh, James K.
中科院分区：
文献类型：
--
作者：
Iwamoto, Jun;Matsumoto, Hideo;Yeh, James K.
本研究的目的是研究维生素K-2和利塞膦酸钠对糖皮质激素（GC）治疗大鼠皮质骨的骨形成和吸收，骨细胞腔隙系统和孔隙率的影响。根据以下给药方案，将49只3月龄雌性Sprague-Dawley大鼠随机分为5组：年龄匹配对照组、GC给药组和GC给药联合维生素K-2、利塞膦酸盐或维生素K-2 +利塞膦酸盐给药组。在8周实验结束时，进行经典的骨组织形态计量学分析，并在胫骨骨干的皮质骨上评价骨细胞陷窝系统和孔隙度。由于骨膜骨形成减少、皮质内骨侵蚀增加和皮质孔隙率增加，GC给药降低了皮质骨面积百分比，增加了骨髓面积百分比。维生素K-2可防止骨膜骨形成减少，但不影响皮质骨和骨髓面积百分比。利塞膦酸钠可防止骨膜骨形成减少和皮质内骨侵蚀增加，从而防止皮质骨和骨髓面积百分比的改变。维生素K-2和利塞膦酸钠均增加骨细胞密度和骨陷窝占用率，并防止GC诱导的皮质孔隙率增加。维生素K-2和利塞膦酸钠对骨细胞密度和骨陷窝占有率有相加作用，对皮质骨孔隙率有协同作用。本研究显示了维生素K-2和利塞膦酸钠对骨形成和骨吸收、骨细胞腔隙系统和GC处理大鼠皮质骨孔隙率的功效。
The purpose of the present study was to examine the effects of vitamin K-2 and risedronate on bone formation and resorption, the osteocyte lacunar system, and porosity in the cortical bone of glucocorticoid (GC)-treated rats. Forty-nine female Sprague-Dawley rats, 3 months of age, were randomized into five groups according to the following treatment schedule: age-matched control, GC administration, and GC administration with concomitant administration of vitamin K-2, risedronate, or vitamin K-2 + risedronate. At the end of the 8-week experiment, classical bone histomorphometric analysis was performed, and the osteocyte lacunar system and porosity were evaluated on the cortical bone of the tibial diaphysis. GC administration decreased percent cortical bone area and increased percent marrow area as a result of decreased periosteal bone formation, and increased endocortical bone erosion, and increased cortical porosity. Vitamin K-2 prevented a reduction in periosteal bone formation but did not affect percent cortical bone and marrow areas. Risedronate prevented a reduction in periosteal bone formation and an increase in endocortical bone erosion, resulting in prevention of alterations in percent cortical bone and marrow areas. Both vitamin K-2 and risedronate increased osteocyte density and lacunar occupancy and prevented a GC-induced increase in cortical porosity. Vitamin K-2 and risedronate had additive effects on osteocyte density and lacunar occupancy and a synergistic effect on cortical porosity. The present study showed the efficacy of vitamin K-2 and risedronate for bone formation and resorption, the osteocyte lacunar system, and porosity in the cortical bone of GC-treated rats.