Prolactin release from MtTW15 and 7315a pituitary tumors is refractory to TRH and VIP stimulation.
Prolactin release from MtTW15 and 7315a pituitary tumors is refractory to TRH and VIP stimulation.
复制标题
MtTW15 和 7315a 垂体瘤释放的催乳素对 TRH 和 VIP 刺激无效。
DOI：
10.1016/0303-7207(84)90039-x
复制
发表时间：
1984
影响因子：
4.1
通讯作者：
MacLeod,RM
中科院分区：
文献类型：
--
作者：
Judd,AM;Canonico,PL;MacLeod,RM
我们研究了催乳素分泌的MtTW 15和7315 a垂体瘤细胞的体外反应性的刺激所选择的促分泌素使用灌流技术。这些细胞的催乳素释放对促甲状腺激素释放激素（TRH）和血管活性肠肽（VIP）是无效的。相反，50 mM K+，二丁酰cAMP，茶碱，磷脂酶A2和佛波酯肉豆蔻酸酯乙酸酯都增加催乳素释放从两种肿瘤细胞类型。磷脂酶C增加催乳素释放从7315 a，但不是从MtTW 15细胞。TRH可增加7315 a细胞~（32）P掺入磷脂酰肌醇的量，但对MtTWIS细胞无此作用。因此，这些肿瘤对TRH和VIP的难治性可能至少部分是由于受体缺陷或受体结合和细胞内生化过程耦合过程中的缺陷。在MtTW 15肿瘤中，至少部分缺陷可能与磷脂水解有关。
We studied the in vitro responsiveness of prolactin-secreting MtTW15 and 7315a pituitary tumor cells to stimulation by selected secretagogues using a perifusion technique. Prolactin release by these cells was refractory to thyrotropin-releasing hormone (TRH) and vasoactive intestinal peptide (VIP). In contrast, 50 mM K+, dibutyryl cAMP, theophylline, phospholipase A2and phorbol myristate acetate all increased prolactin release from both tumor cell types. Phospholipase C increased prolactin release from 7315a but not from MtTW15 cells. TRH increased32P incorporation into phosphatidylinositol in the 7315a but not in the MtTWIS tumor cells. Therefore, the refractoriness of these tumors to TRH and VIP may be at least partially due to a defect in the receptor or in the process that couples receptor binding and intracellular biochemical processes. In the MtTW15 tumor at least part of the defect may be related to phospholipid hydrolysis.