Stress-induced susceptibility to bacterial infection during cutaneous wound healing
Stress-induced susceptibility to bacterial infection during cutaneous wound healing
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DOI：
10.1006/brbi.2000.0619
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发表时间：
2002-02-01
影响因子：
15.1
通讯作者：
Marucha, PT
中科院分区：
文献类型：
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作者：
Rojas, IG;Padgett, DA;Marucha, PT
心理压力会延迟伤口愈合，降低细菌清除所需的免疫/炎症反应。为了确定应激是否增加，对伤口感染的易感性，在放置皮肤伤口之前3天开始，使雌性SKH-1小鼠经受束缚应激（restraint stress，REH）。从收获的伤口定量活细菌。抗生素使愈合延迟30%，并导致机会性细菌（例如，金黄色葡萄球菌），（p <0.05）。到第7天，85.4%的实验小鼠伤口具有预测感染的细菌计数，而对照小鼠为27.4%（p <0.001）。为了评估RST诱导的糖皮质激素在细菌清除中的作用，用糖皮质激素受体拮抗剂RU 486处理小鼠。RU 486在来自H2 O2小鼠的伤口中将机会性细菌减少了近1个对数（p <0.05）。因此，应力损害伤口愈合期间的细菌清除，导致机会性感染的发生率显著增加。(C)2001 Elsevier Science（美国）。
Psychological stress delays wound healing and decreases immune/inflammatory responses required for bacterial clearance. To determine if stress increase, the susceptibility to wound infection, female SKH-1 mice were subjected to restraint stress (RST) beginning 3 days prior to the placement of cutaneous wounds. Viable bacteria were quantified from harvested wounds. RST delayed healing by 30% and caused a 2- to 5-log increase in opportunistic bacteria (e.g., Staphylococcus aureus) when compared to wounds from control animal, (p < .05). By day 7, 85.4% of the wounds from RST mice had bacterial counts predictive of infection compared to 27.4% from control mice (p < .001). To assess the role of RST-induced glucocorticoids in bacterial clearance, mice were treated with the glucocorticoid receptor antagonist RU486. RU486 reduced opportunistic bacteria by nearly 1 log in wounds from RST mice (p < .05). Thus, stress impairs, bacterial clearance during wound healing, resulting in a significant increase in the incidence of opportunistic infection. (C) 2001 Elsevier Science (USA).