Response of the antioxidant enzymes of rats following oral administration of metal-oxide nanoparticles (Al2O3, CuO, TiO2)
Response of the antioxidant enzymes of rats following oral administration of metal-oxide nanoparticles (Al2O3, CuO, TiO2)
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DOI：
10.1007/s11356-018-3592-8
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Canli, Mustafa
中科院分区：
文献类型：
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作者：
Canli, Esin G.;Ila, Hasan B.;Canli, Mustafa
金属氧化物纳米颗粒作为一种新兴的技术化合物，具有广泛的应用前景，同时也具有潜在的环境毒理学效应。在本工作中，铝（Al 2 O3），铜（CuO），和钛（TiO 2）纳米粒子（NPs）通过口服灌胃给药到成熟的雌性大鼠（Rattus norvegicus var.白化病人），连续给药14天，剂量系列为0（对照）、0.5、5和50（mg/kg b.w./天）。测定肝脏中抗氧化系统的酶活性，如过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）和谷胱甘肽还原酶（GR）。取肝脏的透射电子显微镜（TEM）图像以证明NP在肝组织中的积累和分布。结果表明，各纳米颗粒均引起抗氧化酶活性的显著变化（P>0.05）。CuO和TiO 2处理后CAT活性升高，而Al 2 O3处理后SOD活性降低。与谷胱甘肽（GR，GPx，GST）代谢相关的酶的活性也被NP显著改变。Al_2O_3、CuO纳米粒均能提高GPx活性，而GR活性仅能提高Al_2O_3。然而，有增加（TiO 2）和减少（CuO）的GST活性在大鼠的肝脏。肝脏的TEM图像表明，即使在最低剂量下，所有NP也在肝脏中积累。本研究表明，大鼠肝脏中的抗氧化酶的影响，由所有NP，表明大鼠的抗氧化系统受到损害后，NP的管理。
Metal-oxide nanoparticles (NPs), as a new emerging technological compound, promise a wide range of usage areas and consequently have the potential to cause environmental toxicology. In the present work, aluminum (Al2O3), copper (CuO), and titanium (TiO2) nanoparticles (NPs) were administered via oral gavage to mature female rats (Rattus norvegicus var. albinos) for 14days with a dose series of 0 (control), 0.5, 5, and 50 (mg/kg b.w./day). Enzyme activities of the antioxidant system such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and glutathione reductase (GR) in the liver were measured. Transmission electron microscope (TEM) images of the liver were taken to demonstrate NP accumulation and distribution in liver tissue. Data showed that all NPs caused some significant (P>0.05) alterations in the activities of antioxidant enzymes. CAT activity increased after CuO and TiO2 administrations, while SOD activity decreased after Al2O3 administration. The activities of enzymes associated with glutathione (GR, GPx, GST) metabolisms were also significantly altered by NPs. GPx activity increased in rats received Al2O3, CuO NPs, while GR activity increased only by Al2O3. However, there were increases (TiO2) and decreases (CuO) in GST activity in the liver of rats. TEM images of the liver demonstrated that all NPs accumulated in the liver, even at the lowest dose. This study indicated that the antioxidant enzymes in the liver of rats were affected by all NPs, suggesting the antioxidant system of rats suffered after NP administration.