Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers
Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers
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DOI：
10.1016/s0021-9673(01)00806-8
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发表时间：
2001-08-10
影响因子：
4.1
通讯作者：
Hostettmann, K
中科院分区：
文献类型：
--
作者：
Waridel, P;Wolfender, JL;Hostettmann, K
LC-MS-MS已成为植物粗提取物中天然产物在线鉴定的重要工具。为了在天然产物的分离中有效地利用该技术，需要仔细研究用于生成信息丰富的MS-MS谱的参数。本文采用混合四极杆飞行时间和离子阱（IT）质谱仪，系统研究了不同CID能量条件下普遍存在的c -糖苷类黄酮的碰撞诱导解离（CED）质谱。基于特定c -糖苷单元片段的CID-MS-MS光谱比较，可以有效地区分类黄酮c -糖苷异构体。6-C和8-C黄酮类苷在x -0,2（+）片段（[M+H-120](+)）的产物离子光谱中观察到显著差异。给出了LC-MS-MS或lc -多阶段MS在线表征c -糖苷类黄酮的指导原则。(C) 2001 Elsevier Science B.V.版权所有
LC-MS-MS is becoming a very important tool for the on-line identification of natural products in crude plant extracts. For an efficient use of this technique in the dereplication of natural products, a careful study of the parameters used to generate informative MS-MS spectra is needed. In this paper, the collision-induced dissociation (CED) MS-MS spectra of ubiquitous C-glycosidic flavonoids have been systematically studied using hybrid quadrupole time-of-flight and ion-trap (IT) mass analysers under various CID energy conditions. Efficient differentiation of flavonoid C-glycoside isomers was possible, based on the comparison of CID-MS-MS spectra of particular C-glycoside unit fragments. Striking differences between 6-C and 8-C flavonoid glycosides were especially observed in the product ion spectra of their X-0,2(+) fragments ([M+H-120](+)). Some guidelines for the on-line characterisation of C-glycosidic flavonoids by LC-MS-MS or LC-multiple-stage MS are given. (C) 2001 Elsevier Science B.V. All rights reserved.