Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial.
Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial.
复制标题
微电极阵列-评估镁作为可植入生物材料的神经毒性作用
DOI：
10.1016/j.jmst.2015.08.009
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发表时间：
2016-01-01
影响因子：
10.9
通讯作者：
Gao BZ
中科院分区：
文献类型：
--
作者：
Huang T;Wang Z;Wei L;Kindy M;Zheng Y;Xi T;Gao BZ
镁基生物材料在临床应用中显示出巨大的潜力。然而，过量的Mg 2+和镁基生物材料的腐蚀产物的细胞毒性效应，特别是它们对神经元的影响，很少有研究。虽然可行性测试是最常用的，功能评估是至关重要的。在这里，使用甲基噻唑基四唑（MTT）和乳酸脱氢酶（LDH）测定来测试Mg 2+和Mg-提取液对神经元活力的影响。微电极阵列（MEAs）可长期、实时记录体外神经元网络的细胞外电生理信号，用于测试毒性作用。MTT法和LDH法测得的Mg 2+最小有效浓度（ECmin）分别为3 mmol/L和100 mmol/L，而MEA法测得的ECmin为0.1 mmol/L。MEA数据显示，当培养物暴露于25%的Mg提取物溶液时，神经元网络活性显著丧失，该浓度不影响神经元活力。对于评估镁基生物材料与神经元的生物相容性，MEA电生理测试是比基本细胞活力测试更精确的方法。
Magnesium (Mg)-based biomaterials have shown great potential in clinical applications. However, the cytotoxic effects of excessive Mg2+ and the corrosion products from Mg-based biomaterials, particularly their effects on neurons, have been little studied. Although viability tests are most commonly used, a functional evaluation is critically needed. Here, both methyl thiazolyl tetrazolium (MTT) and lactate dehydrogenase (LDH) assays were used to test the effect of Mg2+ and Mg-extract solution on neuronal viability. Microelectrode arrays (MEAs), which provide long-term, real-time recording of extracellular electrophysiological signals of in vitro neuronal networks, were used to test for toxic effects. The minimum effective concentrations (ECmin) of Mg2+ from the MTT and LDH assays were 3 mmol/L and 100 mmol/L, respectively, while the ECmin obtained from the MEA assay was 0.1 mmol/L. MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution, a concentration that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons, MEA electrophysiological testing is a more precise method than basic cell-viability testing.