Engineering Functional DNA–Protein Conjugates for Biosensing, Biomedical, and Nanoassembly Applications
Engineering Functional DNA–Protein Conjugates for Biosensing, Biomedical, and Nanoassembly Applications
复制标题
DOI：
10.1007/s41061-020-00305-7
复制
发表时间：
2020-05
影响因子：
8.6
通讯作者：
Dan Zhao;Yuhan Kong;Sisi Zhao;Hang Xing
中科院分区：
文献类型：
--
作者：
Dan Zhao;Yuhan Kong;Sisi Zhao;Hang Xing
DNA和蛋白质是构成生命基础的两类最重要的生物大分子。通过使用交联剂将两者结合在一起，可以实现分子识别、酶催化和沃森-克里克杂交特性的结合。具有综合性能的DNA-蛋白质结合物可以实现广泛的应用，如灵敏和选择性的生物分析、治疗剂和可编程纳米组装的构建块。本文从偶联化学以及在生物医学和纳米技术领域的应用等方面综述了偶联物的研究进展。我们强调了偶联物的生物部分在生物检测中用于靶标结合和信号转导的功能。我们还回顾了DNA-蛋白质偶联物在构建各种功能和动态纳米结构方面的应用，从孤立的杂交笼子到三维(3D)蛋白质晶格。此外，这些偶联物还被用作载体来运送用于疾病治疗和基因编辑的酶或功能核酸。
DNA and protein are the most important two classes of biomacromolecules forming the basis of life. The conjugation of the two using crosslinking chemistries enables a combination of molecular recognition, enzymatic catalysis, and Watson– Crick hybridization properties. The DNA–protein conjugate with combined properties enables a broad range of applications, such as sensitive and selective bioassays, therapeutic agents, and building blocks for programmable nanoassemblies. In this review, we survey the conjugates from the aspects of conjugation chemistries as well as applications in biomedical and nanotechnology fields. We highlight the functions of both biological moieties of a conjugate for target binding and signal transduction in bioassays. We also review the use of DNA–protein conjugates for the construction of a variety of functional and dynamic nanostructures, from isolated hybrid cages to three-dimensional (3D) protein crystalline lattices. Moreover, these conjugates have been used as carriers to deliver enzymes or functional nucleic acids for disease treatments and gene editing.