Castration influences intestinal microflora and induces abdominal obesity in high-fat diet-fed mice.
Castration influences intestinal microflora and induces abdominal obesity in high-fat diet-fed mice.
复制标题
DOI：
10.1038/srep23001
复制
发表时间：
2016-03-10
影响因子：
4.6
通讯作者：
Yamaji R
中科院分区：
文献类型：
--
作者：
Harada N;Hanaoka R;Horiuchi H;Kitakaze T;Mitani T;Inui H;Yamaji R
迟发性性腺功能减退症（即雄激素缺乏症）会增加男性腹部肥胖的风险。这种肥胖的机制尚不清楚。在这里，我们证明了去势后的性腺功能减退导致高脂饮食（HFD）喂养的C57 BL/6 J小鼠腹部肥胖，而不是标准饮食（SD）喂养的C57 BL/6 J小鼠。此外，在用破坏肠道微生物菌群的抗生素处理的小鼠中没有诱导表型。在HFD喂养的小鼠中，去势增加了饲料效率，降低了每次食物摄入的粪便重量。去势也诱导内脏脂肪量的增加，只有在没有抗生素的HFD喂养的小鼠，而皮下脂肪量增加去势无关的抗生素。去势降低了HFD喂养小鼠肠系膜脂肪中脂肪甘油三酯脂肪酶和脂肪敏感脂肪酶的表达，而在抗生素存在下未观察到。去势降低了大腿肌肉（即四头肌和腿筋）质量，升高了空腹血糖水平，并以HFD依赖性方式增加了肝脏甘油三酯水平，而在用抗生素处理的去势小鼠中未观察到这些变化。HFD喂养的去势小鼠粪便中厚壁菌/拟杆菌比例和乳杆菌种类增加。这些结果表明，雄激素（例如睾酮）缺乏可以改变肠道微生物组，并以饮食依赖性方式诱导腹部肥胖。
Late-onset hypogonadism (i.e. androgen deficiency) raises the risk for abdominal obesity in men. The mechanism for this obesity is unclear. Here, we demonstrated that hypogonadism after castration caused abdominal obesity in high-fat diet (HFD)-fed, but not in standard diet (SD)-fed, C57BL/6J mice. Furthermore, the phenotype was not induced in mice treated with antibiotics that disrupt the intestinal microflora. In HFD-fed mice, castration increased feed efficiency and decreased fecal weight per food intake. Castration also induced in an increase of visceral fat mass only in the absence of antibiotics in HFD-fed mice, whereas subcutaneous fat mass was increased by castration irrespective of antibiotics. Castration reduced the expression in the mesenteric fat of both adipose triglyceride lipase and hormone-sensitive lipase in HFD-fed mice, which was not observed in the presence of antibiotics. Castration decreased thigh muscle (i.e. quadriceps and hamstrings) mass, elevated fasting blood glucose levels, and increased liver triglyceride levels in a HFD-dependent manner, whereas these changes were not observed in castrated mice treated with antibiotics. The Firmicutes/Bacteroidetes ratio and Lactobacillus species increased in the feces of HFD-fed castrated mice. These results show that androgen (e.g. testosterone) deficiency can alter the intestinal microbiome and induce abdominal obesity in a diet-dependent manner.