Trypanosoma cruzi:: A considerable phylogenetic divergence indicates that the agent of Chagas disease is indigenous to the native fauna of the United States
Trypanosoma cruzi:: A considerable phylogenetic divergence indicates that the agent of Chagas disease is indigenous to the native fauna of the United States
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DOI：
10.1006/expr.2001.4651
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发表时间：
2001-10-01
影响因子：
2.1
通讯作者：
Tibayrenc, M
中科院分区：
文献类型：
--
作者：
Barnabé, C;Yaeger, R;Tibayrenc, M
采用22个基因位点的多位点酶电泳和10个随机引物的多态性DNA扩增技术，对30个主要来源于野生哺乳动物的克氏锥虫进行了鉴定。两个主要的系统发生簇，由大的遗传距离分开。分别对应于先前描述的酶组I和III。从土著人病例中分离的两个种群被鉴定为zymode I。美国的遗传多样性。克氏分离物相当多。与南美洲类似规模样本的得分相当。这些结果支持的假设，T cruzi美国股票没有进口的历史时间，是本土的本土动物群的美国。这些种群的种群结构似乎基本上是克隆的，正如以前在南美洲报道的那样。在美国没有发现杂交基因型的证据。(C)2001年，爱思唯尔科学。
Thirty U.S. Trypanosoma cruzi stocks isolated mainly from wild mammals were characterized by multilocus enzyme electrophoresis at 22 genetic loci and random amplification of polymorphic DNA for 10 primers. Two main phylogenetic clusters, separated by large genetic distances. were discriminated by both methods, corresponding, respectively, to the formerly described zymodemes I and III. Two stocks isolated from indigenous human cases were identified as zymodeme I. Genetic diversity of the U.S. T. cruzi isolates was considerable. comparable to that scored in similarly sized samples from South America. These results favor the hypothesis that T cruzi U.S. stocks were not imported at a historical time and are indigenous to the native fauna of the United States. The population structure of these stocks appeared to be basically clonal, as previously reported in South America. and no evidence of hybrid genotypes was found in the United States. (C) 2001 Elsevier Science.