Computer simulation of affinity capillary electrophoresis
Computer simulation of affinity capillary electrophoresis
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DOI：
10.1002/1522-2683(200203)23:6
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发表时间：
2002-03-01
期刊：
影响因子：
2.9
通讯作者：
Righetti, PG
中科院分区：
文献类型：
--
作者：
Andreev, VP;Pliss, NS;Righetti, PG
建立了亲和毛细管电泳法的计算机模拟模型。与现有的模型不同，它能够描述样品分子和配体分子的浓度可公度的情况，甚至这些分子占据的区域最初没有混合的情况。该模型可以研究样品分子的时空分布与各种参数的关系，如反应速率常数、样品和试剂的浓度、样品和试剂的电迁移速度以及样品进样量。给出了不同参数值的峰形集合。研究了峰方差与分析时间与特征反应时间之比的关系。
A computer-simulated model of affinity capillary electrophoresis is developed. Unlike existing models, it is able to describe the situation where the concentrations of sample molecules and ligand molecules are commensurable, or even the situation where the zones occupied by these molecules are not mixed initially. The model permits to study the dependence of the spatial and temporal distributions of sample molecules on various parameters such as reaction rate constants, concentrations of sample and reagent, electromigration velocities of sample and reagent and sample injection volume. A collection of peak shapes for different values of parameters is presented. The dependence of peak variance on the ratio of the time of analysis to the characteristic time of reaction is studied.