Characterization of the endoproteases appearing during wheat grain development
Characterization of the endoproteases appearing during wheat grain development
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DOI：
10.1104/pp.112.3.1211
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发表时间：
1996-11-01
期刊：
影响因子：
7.4
通讯作者：
Cejudo, FJ
中科院分区：
文献类型：
--
作者：
Dominguez, F;Cejudo, FJ
研究了小麦（Triticum aestivum，栽培品种中国春）籽粒发育过程中发生的内切蛋白水解活性的模式。在以偶氮酪蛋白为底物的溶液中测定的总内切蛋白酶活性在籽粒发育的早期阶段增加，并在花后15天达到最大值，并一直保持到籽粒成熟。还在与明胶共聚的梯度聚丙烯酰胺凝胶中测定了内切蛋白酶活性。内切蛋白水解活性的增加是由于出现了多达 18 个内切蛋白水解带，这些带被任意分为五组（A、B、C、D 和 E）。通过使用特定类别的蛋白酶抑制剂，证明了发育中的小麦籽粒中存在丝氨酸、天冬氨酸、金属蛋白酶以及较小程度的硫醇蛋白酶。种子发育过程中不同类别内切蛋白酶的出现受到时间控制；丝氨酸蛋白酶在早期阶段更加丰富，天冬氨酸和金属蛋白酶在后期阶段更加丰富。在发育中期（花后 15-20 天），大多数内切蛋白酶位于糊粉层、种皮和胚胎中。发育中的淀粉胚乳中酸性硫醇蛋白酶的含量较低。
The pattern of endoproteolytic activities occurring during wheat (Triticum aestivum, cultivar Chinese Spring) grain development was investigated. Total endoprotease activity, assayed in solution with azocasein as a substrate, increased during the early stages of grain development to reach a maximum at 15 d postanthesis that was maintained until the grain was mature. Endoprotease activity was also assayed in gradient polyacrylamide gels co-polymerized with gelatin. The increase in endoproteolytic activity was due to the appearance of up to 18 endoproteolytic bands that were arbitrarily classified into five groups (A, B, C, D, and E). The presence of serine, aspartic, metallo, and, to a lesser extent, thiol proteases in developing wheat grains was demonstrated by the use of class-specific protease inhibitors. The appearance of the different classes of endoproteases during seed development was subject to temporal control; serine proteases were more abundant at early stages and aspartic and metallo proteases were more abundant at later stages. At intermediate stages of development (15-20 d postanthesis), most of the endoproteases were localized in the aleurone, testa, and embryo. The content of acidic thiol proteases was low in the developing starchy endosperm.