THE CHARACTERIZATION AND MOLECULAR-CLONING OF THE DOUBLE-STRANDED-RNA GENOME OF AN AUSTRALIAN STRAIN OF INFECTIOUS BURSAL DISEASE VIRUS
THE CHARACTERIZATION AND MOLECULAR-CLONING OF THE DOUBLE-STRANDED-RNA GENOME OF AN AUSTRALIAN STRAIN OF INFECTIOUS BURSAL DISEASE VIRUS
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DOI：
10.1016/0042-6822(85)90094-7
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发表时间：
1985-01-01
期刊：
影响因子：
3.7
通讯作者：
FAHEY, KJ
中科院分区：
文献类型：
--
作者：
AZAD, AA;BARRETT, SA;FAHEY, KJ
发现传染性法氏囊病病毒（IBDV）002-73株的基因组由两段双链（ds）RNA组成，它们是3400（bp）（碱基对）（MW 2.06 × 106）。106)和2900 bp（MW 1.76 × 1.76）。106)长，分别。双链IBDV RNA只有在广泛变性后才能在体外翻译。发现小RNA片段编码MW 90 K的单一多肽，而大RNA片段编码MW 52 K、32 K和28 K的3种主要多肽和MW 41 K和16 K的2种次要多肽。大的RNA片段可以编码分子量为125 K的蛋白质，而翻译产物的分子量为169 K，这表明一些翻译产物之间存在着底物-产物关系。描述了一种从大的ds RNA分子合成ds互补DNA的方法。重组菌落的分析表明，覆盖整个IBDV基因组的插入片段被克隆。
The genome of infectious bursal disease virus (IBDV) strain 002-73 was found to consist of 2 segments of double-stranded (ds) RNA which were 3400 (bp) (base pair) (MW 2.06 .times. 106) and 2900 bp (MW 1.76 .times. 106) long, respectively. The ds IBDV RNA could be translated, in vitro, only after extensive denaturation. The small RNA segment was found to code for a single polypeptide of MW 90K, while the large RNA segment coded for 3 major polypeptides of MW 52K [52,000), 32K, and 28K and 2 minor polypeptides of MW 41K and 16K. The large RNA segment could encode proteins of MW 125K while the MW of the translated products was 169K suggesting that a precursor-product relationship exists between some of the translation products. A method is described for the synthesis of ds complementary DNA from large ds RNA molecules. Analyses of recombinant colonies showed that inserts covering the entire IBDV genome were cloned.