Tissue-specific expression of TIPE2 provides insights into its function
Tissue-specific expression of TIPE2 provides insights into its function
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DOI：
10.1016/j.molimm.2010.06.016
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发表时间：
2010-09-01
影响因子：
3.6
通讯作者：
Liu, Suxia
中科院分区：
文献类型：
--
作者：
Zhang, Guizhong;Hao, Chunyan;Liu, Suxia
肿瘤坏死因子-α诱导蛋白-8样蛋白-2（TNFAIP812 TIPE2）是一种新发现的先天免疫和细胞免疫的负调节因子。小鼠中TIPE 2缺乏引起胎儿炎性疾病，人类中TIPE 2下调与全身性自身免疫有关。然而，TIPE 2缺乏导致体液免疫的选择性缺陷。在本研究中，我们制备了一种高度特异性的TIPE 2抗体，并通过免疫组织化学和RT-PCR检测了TIPE 2在各种小鼠组织中的表达。淋巴组织在淋巴区室内，T细胞似乎表达高水平的TIPE 2蛋白，而淋巴器官的B细胞和B细胞区缺乏TIPE 2。淋巴组织中未检测到TIPE 2，然而，一些内分泌组织和骨骼肌表达可检测到的TIPE 2蛋白和mRNA。此外，在单核细胞/巨噬细胞衍生的细胞系和卵巢腺癌细胞中检测到高水平的TIPE 2，但在大多数人癌细胞系中检测不到或弱表达。淋巴隔室它们也可以解释为什么TIPE 2缺乏增强细胞免疫而不是体液免疫（C）2010 Elsevier Ltd版权所有
Tumor necrosis factor-alpha-Induced protein-8 like-2 (TNFAIP812 TIPE2) is a newly discovered negative regulator of innate immunity and cellular immunity TIPE2 deficiency in mice causes fetal inflammatory diseases and TIPE2 downregulation in humans is associated with systemic autoimmunity However TIPE2 deficiency leads to a selective defect in humoral immunity Due to the lack of a suitable antibody the nature of cells and tissues that express TIPE2 protein has not been determined In this study we generated a highly specific antibody to TIPE2 and examined TIPE2 expression in various murine tissues by immunohistochemistry and RT-PCR We found that TIPE2 was a cytoplasmic protein expressed preferentially in lymphoid tissues and a small group of non-lymphoid tissues Within the lymphoid compartment T cells appear to express high level of TIPE2 protein while B cells and B cell zones of lymphoid organs were devoid of TIPE2 Within most of the non-lymphoid tissues TIPE2 was not detected However several endocrine tissues and skeletal muscle expressed detectable TIPE2 protein and mRNA Furthermore high levels of TIPE2 were detected in monocyte/macrophage derived cell lines and ovarian adenocarcinoma cells but not detectable or weakly expressed in most human carcinoma cell lines These results indicate that TIPE2 may perform tissue-specific functions in both lymphoid and non-lymphoid compartments They may also explain why TIPE2 deficiency enhanced cellular but not humoral immunity (C) 2010 Elsevier Ltd All rights reserved