Antibody to threonyl-transfer RNA synthetase in myositis sera.
Antibody to threonyl-transfer RNA synthetase in myositis sera.
复制标题
肌炎血清中苏氨酰转移 RNA 合成酶的抗体。
DOI：
10.1002/art.1780310408
复制
发表时间：
1988
影响因子：
--
通讯作者：
M. Reichlin
中科院分区：
文献类型：
--
作者：
I. Targoff;I. Targoff;F. Arnett;M. Reichlin;M. Reichlin
在 109 份肌炎患者血清中研究了抗苏氨酰转移 RNA 合成酶（抗 PL-7）的患病率和临床相关性，以及抗 PL-7 与抗组氨酰转移 RNA 合成酶（抗 Jo-1）的关系。使用苏氨酸氨酰化的抑制来筛选抗PL-7。来自 3 名患者的血清，其中 2 名患有多发性肌炎，1 名患有多发性肌炎重叠综合征，以及来自皮肌炎患者的第四份血清（之前发现含有抗 PL-7），抑制了超过 90% 的活性（109 份血清中的 3.7%）。所有 4 种血清在酶联免疫吸附测定中均与用抗 PL-7 亲和纯化或生化纯化的酶发生强烈反应。氨酰化抑制或大多数血清的酶联免疫吸附测定均未显示交叉反应性。抗 PL-7 是一种罕见的肌炎相关抗体，独立于抗 Jo-1，但针对功能相关的酶。
The prevalence and clinical correlations of anti-threonyl-transfer RNA synthetase (anti-PL-7), as well as the relationship of anti-PL-7 to anti-histidyl-transfer RNA synthetase (anti-Jo-1) were studied in 109 sera from patients with myositis. Inhibition of threonine aminoacylation was used to screen for anti-PL-7. Sera from 3 patients, 2 with polymyositis and 1 with polymyositis-overlap syndrome, and a fourth serum from a patient with dermatomyositis, which was previously found to contain anti-PL-7, inhibited greater than 90% of activity (3.7% of 109 sera). All 4 sera reacted strongly in an enzyme-linked immunosorbent assay with enzyme that was either affinity purified with anti-PL-7 or was biochemically purified. There was no indication of cross-reactivity by aminoacylation inhibition or, for most sera, by enzyme-linked immunosorbent assay. Anti-PL-7 is an uncommon myositis-associated antibody that is independent of anti-Jo-1, but is directed at a functionally related enzyme.
多发性肌炎/皮肌炎中的 Jo-1 抗体：与间质性肺病的关联。
DOI：
--
发表时间：
1984
期刊：
The Journal of rheumatology
影响因子：
--
作者：
Hochberg,MC;Feldman,D;Stevens,MB;Arnett,FC;Reichlin,M
通讯作者：
Reichlin,M