Glycerol regulates stratum corneum hydration in sebaceous gland deficient (Asebia) mice
Glycerol regulates stratum corneum hydration in sebaceous gland deficient (Asebia) mice
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DOI：
10.1046/j.1523-1747.2003.12134.x
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发表时间：
2003-05-01
影响因子：
6.5
通讯作者：
Elias, PM
中科院分区：
文献类型：
--
作者：
Fluhr, JW;Mao-Qiang, M;Elias, PM
唯一已知的人类皮脂腺的功能是痤疮的挑衅。我们在此评估皮脂是否影响严重皮脂腺发育不全的asbia J1和2j小鼠角质层水化或通透屏障功能。Asebia J1小鼠表现出正常的渗透性屏障稳态和细胞外板层膜结构，但它们表现出表皮增生、炎症和角质层水化减少（约50%），与皮脂腺脂质（蜡酯/单酯、甾醇酯）减少有关。asbia和对照角质层的甘油三酯含量都很低，这与正常毛囊皮脂腺内甘油三酯水解率高一致，尽管甘油三酯合成率高。虽然合成的皮脂样脂类混合物（固醇/蜡酯、甘油三酯）不能使无痛皮肤恢复正常的角质层水合作用，但外用甘油（皮脂腺中甘油三酯水解的推定产物）、正常角质层水合作用和无痛皮肤角质层甘油含量比正常角质层低85%。相比之下，另一种有效的内源性湿润剂（尿素）没有纠正这种异常。皮脂腺中甘油三酯生成甘油对角质层水化的重要性进一步得到了证明：(1)在asbia小鼠中缺乏皮脂腺相关脂肪酶活性，而在对照小鼠的腺体中存在丰富的酶活性；（ii）高浓度外用甘油三酯无法纠正水化异常，尽管在兽皮角质层中存在丰富的脂肪酶活性。这些结果表明，皮脂腺衍生的甘油是角质层水化的主要贡献者。
The only known function of human sebaceous glands is the provocation of acne. We assessed here whether sebum influences stratum corneum hydration or permeability barrier function in asebia J1 and 2 J mice, with profound sebaceous gland hypoplasia. Asebia J1 mice showed normal permeability barrier homeostasis and extracellular lamellar membrane structures, but they displayed epidermal hyperplasia, inflammation, and decreased (>50%) stratum corneum hydration, associated with a reduction in sebaceous gland lipids (wax diesters/monoesters, sterol esters). The triglyceride content of both asebia and control stratum corneum was low, consistent with high rates of triglyceride hydrolysis within the normal pilosebaceous apparatus, despite high rates of triglyceride synthesis. Although a mixture of synthetic, sebum-like lipids (sterol/wax esters, triglycerides) did not restore normal stratum corneum hydration to asebia skin, topical glycerol, the putative product of triglyceride hydrolysis in sebaceous glands, normalized stratum corneum hydration, and the glycerol content of asebia stratum corneum was 85% lower than in normal stratum corneum. In contrast, another potent endogenous humectant (urea) did not correct the abnormality. The importance of glycerol generation from triglyceride in sebaceous glands for stratum corneum hydration was demonstrated further by (i) the absence of sebaceous-gland-associated lipase activity in asebia mice, whereas abundant enzyme activity was present in the glands of control mice; and (ii) the inability of high concentrations of topical triglyceride to correct the hydration abnormality, despite the presence of abundant lipase activity in asebia stratum corneum. These results show that sebaceous-gland-derived glycerol is a major contributor to stratum corneum hydration.