AN NK1.1+ CD4+8- SINGLE-POSITIVE THYMOCYTE SUBPOPULATION THAT EXPRESSES A HIGHLY SKEWED T-CELL ANTIGEN RECEPTOR-V-BETA FAMILY
AN NK1.1+ CD4+8- SINGLE-POSITIVE THYMOCYTE SUBPOPULATION THAT EXPRESSES A HIGHLY SKEWED T-CELL ANTIGEN RECEPTOR-V-BETA FAMILY
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DOI：
10.1073/pnas.89.14.6506
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发表时间：
1992-07-15
影响因子：
11.1
通讯作者：
ONOE, K
中科院分区：
文献类型：
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作者：
ARASE, H;ARASE, N;ONOE, K
在本报告中，我们描述了一个CD 4 +8-热稳定抗原阴性（HSA-）胸腺细胞亚群，表达了一个相当低密度的α-β-T细胞抗原受体（TCR（lo）），从大多数的CD 4 +8-高密度TCR（TCR（hi））成熟型胸腺细胞。这个亚群出现在相对较晚的生活。对该亚群的MEL-14、Pgp-1（CD 44）、ICAM-1（CD 54）和NK1.1表达的分析显示，CD 4 +8- TCR（lo）群体是具有独特特征的群体与CD 4 +8- TCR（hi）胸腺细胞相比，其大多数是MEL-14+、CD 44-、ICAM-1-和NK1.1-。当分析TCR β链可变区（Vp）的使用时，该胸腺群体主要表达V（β-7）和V（β-8.2）TCR基因家族的产物。有趣的是，具有V（β-8.1）TCR的细胞（其对Mls-1a抗原具有反应性）未从CD 4 +8-HSA-TCR（lo）亚群中消除，但已从Mls-1a菌株中的主要CD 4 +8-HSA-TCR（hi）亚群中消除.还主要在骨髓中鉴定出具有与CD 4 +8-HSA-TCR（lo）胸腺细胞相似的表型的亚群，并且该亚群构成骨髓中约一半的CD 4 + T细胞。CD 4 +8-HSA-TCR（lo）细胞对固定的抗TCR抗体显示出极高的增殖应答，但与主要的CD 4 +8-HSA-CD 3（hi）细胞产生的应答相比，对同种异体H-2抗原产生的应答可忽略不计.然而，Mls-1b小鼠中的CD 4 +8-HSA-TCR（lo）细胞对Mls-1a抗原产生强烈的增殖反应，而Mls-1a小鼠中没有。该T细胞亚群的性质表明这些细胞属于与主要T细胞群体不同的谱系。
In the present report we describe a CD4+8- heat stable antigen-negative (HSA-) thymocyte subpopulation that expresses a distinguishably low density of alpha-beta-T-cell antigen receptors (TCR(lo)) from the majority of CD4+8- high-density TCR (TCR(hi)) mature-type thymocytes. This subpopulation appears relatively late in life. Analysis of MEL-14, Pgp-1 (CD44), ICAM-1 (CD54), and NK1.1 expression on this subpopulation revealed that the CD4+8- TCR(lo) population was a population having unique characteristics (MEL-14-, CD44+, ICAM-1+, and NK1.1+) compared to the CD4+8- TCR(hi) thymocytes, most of which are MEL-14+, CD44-, ICAM-1-, and NK1.1-. When TCR beta-chain variable region (Vp) usage was analyzed, this thymic population expressed predominantly products of V(beta-7) and V(beta-8.2) TCR gene families. Interestingly, cells with V(beta-8.1) TCRs, which are reactive to Mls-1a antigens, were not eliminated from the CD4+8- HSA- TCR(lo) subpopulation but had been eliminated from the major CD4+8- HSA- TCR(hi) subpopulation in Mls-1a strains. A subset with a phenotype similar to the CD4+8- HSA- TCR(lo) thymocytes was also identified primarily in bone marrow, and this subset constituted approximately half of the CD4+ T cells in the bone marrow. The CD4+8- HSA- TCR(lo) cells showed extremely high proliferative responses to immobilized anti-TCR antibody but generated negligible responses to allogeneic H-2 antigens compared to the responses generated by the major CD4+8- HSA- CD3(hi) cells. However, the CD4+8- HSA- TCR(lo) cells in Mls-1b mice mounted vigorous proliferative responses to Mls-1a antigens but not in Mls-1a mice. The properties of this T-cell subset suggest that these cells belong to a lineage distinct from the major T-cell population.