Reconstruction of large cranial defects in nonimmunosuppressed experimental design with human dental pulp stem cells
Reconstruction of large cranial defects in nonimmunosuppressed experimental design with human dental pulp stem cells
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DOI：
10.1097/scs.0b013e31815c8a54
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发表时间：
2008-01-01
影响因子：
0.9
通讯作者：
Passos-Bueno, Maria Rita
中科院分区：
文献类型：
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作者：
Costa, Andre de Mendona;Bueno, Daniela F.;Passos-Bueno, Maria Rita
本研究的主要目的是评估从乳牙中分离的人牙髓干细胞（hDPSC）在非免疫抑制（NIS）大鼠中重建大尺寸颅骨缺损的能力。据我们所知，这些细胞以前没有用于类似的实验。我们进行了两个对称的全层颅骨缺损（5 × 8毫米）的每个顶骨区的八个NIS大鼠。其中6例，左侧仅供应胶原膜，右侧（RS）供应胶原膜和hDPSC。在两只大鼠中，RS仅具有胶原蛋白膜，左侧未添加任何物质（对照）。细胞在体外表征为间充质细胞后使用。在术后7、20、30、60和120天对动物实施安乐死，并从缺损处采集颅骨组织样本进行组织学分析。通过分子分析证实了新骨中存在人类细胞的分析。hDPSC谱系对于测试的四种间充质细胞标志物是阳性的，并且在体外显示成骨分化、成脂分化和成肌分化。术后1个月，我们观察到两侧的骨形成，但RS中存在更成熟的骨。人类DNA仅在RS进行了聚合酶链反应扩增，这表明新骨具有人类细胞。在NIS大鼠中使用hDPSC没有引起任何移植。排斥反应我们的研究结果表明，hDPSC是一种额外的细胞资源，用于纠正大鼠的大颅骨缺损，并构成了一个有前途的模型，用于重建人类的大颅骨缺损，在颅面外科。
The main aim of this study is to evaluate the capacity of human dental pulp stem cells (hDPSC), isolated from deciduous teeth, to reconstruct large-sized cranial bone defects in nonimmunosuppressed (NIS) rats. To our knowledge, these cells were not used before in similar experiments. We performed two symmetric full-thickness cranial defects (5 x 8 mm) on each parietal region of eight NIS rats. In six of them, the left side was supplied with collagen membrane only and the right side (RS) with collagen membrane and hDPSC. In two rats, the RS had collagen membrane only and nothing was added at the left side (controls). Cells were used after in vitro characterization as mesenchymal cells. Animals were euthanized at 7, 20, 30, 60, and 120 days postoperatively and cranial tissue samples were taken from the defects for histologic analysis. Analysis of the presence of human cells in the new bone was confirmed by molecular analysis. The hDPSC lineage was positive for the four mesenchymal cell markers tested and showed osteogenic, adipogenic, and myogenic in vitro differentiation. We observed bone formation 1 month after surgery in both sides, but a more mature bone was present in the RS. Human DNA was polymerase chain reaction-amplified only at the RS, indicating that this new bone had human cells. The us e of hDPSC in NIS rats did not cause any graft. rejection. Our findings suggest that hDPSC is an additional cell resource for correcting large cranial defects in rats and constitutes a promising model for reconstruction of human large cranial defects in craniofacial surgery.