Electrospray mass spectra of three proprietary detergents
Electrospray mass spectra of three proprietary detergents
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DOI：
10.1006/abio.2000.4734
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发表时间：
2000-10-15
影响因子：
2.9
通讯作者：
Fales, HM
中科院分区：
文献类型：
--
作者：
Yang-Boja, E;DeFilippes, F;Fales, HM
我们使用电喷雾质谱法测定了 Pierce Chemical Co. 的市售试剂 M-PER、Y-PER 和 B-PER 的主要成分。这三种专有试剂已在生物化学界广泛用作蛋白质纯化初始步骤中的细胞膜溶解工具。然而，这些试剂的身份和机制仍然未知。在本文中，我们将这些试剂分别鉴定为3-[(3-胆酰胺丙基)二甲基铵基]-1-丙烷磺酸盐、N-十四烷基-N,N-二甲基-3-氨基-1-丙磺酸盐和正辛基-β-D-吡喃硫代葡萄糖苷。此外，我们希望在此强调电喷雾质谱在分析此类专有生物制剂中的作用越来越重要，这些生物制剂越来越多地进入生化文献。
We have determined the major ingredients of the commercially available reagents M-PER, Y-PER, and B-PER from Pierce Chemical Co. using electrospray mass spectrometry. These three proprietary reagents have been widely used in the biochemical community as cell membrane dissolving tools during the initial step of protein purification. However, the identity and mechanism of these reagents remained unknown. In this paper, we identified these reagents as 3-[(3-cholamidopropyl) dimethylammonio]-l-propanesulfonate, N-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and n-octyl-beta -D-thioglucopyranoside, respectively. In addition, we wish to stress here the increasing importance of the role of electrospray mass spectrometry in the analysis of such proprietary biological preparations which are increasingly finding their way into the biochemical literature.