Metabolism of anthocyanins and their phenolic degradation products by the intestinal microflora
Metabolism of anthocyanins and their phenolic degradation products by the intestinal microflora
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DOI：
10.1016/j.bmc.2005.05.003
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发表时间：
2005-09-01
影响因子：
3.5
通讯作者：
Humpf, HU
中科院分区：
文献类型：
--
作者：
Keppler, K;Humpf, HU
花青素被认为是负责对心血管疾病和某些形式的癌症的保护作用。虽然以前的研究表明，完整的花青素苷在胃肠道中的相互作用被广泛减少，只有很少的数据是关于肠道微孔的代谢。使用一种新的体外模型，我们已经研究了微生物去糖基化和降解的六个花色苷表现出三种不同的糖苷配基与单或二-β-D-糖苷键，使用高效液相色谱-二极管阵列（HPLC-DAD）和气相色谱-质谱（GC-MS）检测。我们已经发现，所有的花色素苷水解的微生物群在20分钟和2小时的孵育取决于糖部分。由于释放的糖苷配基在中性pH下的高度不稳定性，在孵育20分钟后已经检测到初级酚类降解产物。酚酸的进一步代谢伴随着脱甲基作用。由于其较高的化学和微生物稳定性，酚酸和/或其他尚未鉴定的花青素代谢物可能是体内观察到的抗氧化活性和其他生理效应的主要原因。(c)2005爱思唯尔有限公司保留所有权利。
Anthocyanins are suggested to be responsible for protective effects against cardiovascular diseases and certain forms of cancer. Although previous studies have implicated that intact anthocyanidin glycosides were decreased extensively by interactions in the gastrointestinal tract, only few data are available concerning the metabolism by the intestinal microffora. Using a new in vitro model, we have investigated the microbial deglycosylation and degradation of six anthocyanins exhibiting three different aglycones with mono- or di-beta-D-glycosidic bonds using high-performance liquid chromatography-diode array (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS) detection. We have found that all anthocyandin glycosides were hydrolysed by the microflora within 20 min and 2 h of incubation depending on the sugar moiety. Due to the high instability of the liberated aglycones at neutral pH, primary phenolic degradation products were already detected after 20 min of incubation. Further metabolism of the phenolic acids was accompanied by demethylation. Because of their higher chemical and microbial stability, phenolic acids and/or other, not yet identified, anthocyanin metabolites might be mainly responsible for the observed antioxidant activities and other physiological effects in vivo. (c) 2005 Elsevier Ltd. All rights reserved.