Inhaled corticosteroid use is associated with increased circulating T regulatory cells in children with asthma.
Inhaled corticosteroid use is associated with increased circulating T regulatory cells in children with asthma.
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DOI：
10.1186/1476-7961-11-1
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发表时间：
2013-01-25
期刊：
影响因子：
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通讯作者：
Seroogy CM
中科院分区：
文献类型：
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作者：
Singh AM;Dahlberg P;Burmeister K;Evans MD;Gangnon R;Roberg KA;Tisler C;Dasilva D;Pappas T;Salazar L;Lemanske RF Jr;Gern JE;Seroogy CM
T调节性（Treg）细胞在平衡免疫反应中起重要作用，Treg细胞的失调与包括哮喘在内的多种疾病的发病机制有关。在这项研究中，我们的主要目的是确定哮喘儿童和非哮喘儿童外周血中Treg细胞的频率。第二个目的是探索Treg细胞频率与过敏原致敏、疾病严重程度和药物使用之间的关系。采用多参数流式细胞术对健康对照者（93例）和不同疾病严重程度的哮喘患儿（66例）外周血单核细胞进行了检测。我们的研究结果表明，与没有哮喘的儿童相比，哮喘儿童的Treg细胞频率显著增加。使用多变量模型，哮喘儿童Treg细胞频率增加与吸入皮质类固醇使用最直接相关，而与哮喘严重程度、过敏致敏或哮喘的特应性状态无关。我们的结论是，低剂量的局部气道给药皮质类固醇足以影响外周血中Treg细胞的频率。这些数据强调了在研究Treg细胞时考虑药物暴露的重要性，并提示哮喘患者吸入皮质类固醇可通过增加Treg细胞频率来改善疾病控制。
T regulatory (Treg) cells are important in balancing immune responses and dysregulation of Treg cells has been implicated in the pathogenesis of multiple disease states including asthma. In this study, our primary aim was to determine Treg cell frequency in the peripheral blood of children with and without asthma. The secondary aim was to explore the association between Treg cell frequency with allergen sensitization, disease severity and medication use. Peripheral blood mononuclear cells from healthy control subjects (N = 93) and asthmatic children of varying disease severity (N = 66) were characterized by multi-parameter flow cytometry. Our findings demonstrate that children with asthma had a significantly increased frequency of Treg cells compared to children without asthma. Using a multivariate model, increased Treg cell frequency in children with asthma was most directly associated with inhaled corticosteroid use, and not asthma severity, allergic sensitization, or atopic status of the asthma. We conclude that low dose, local airway administration of corticosteroids is sufficient to impact the frequency of Treg cells in the peripheral blood. These data highlight the importance of considering medication exposure when studying Treg cells and suggest inhaled corticosteroid use in asthmatics may improve disease control through increased Treg cell frequency.