Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin:: Time-sequence observations of the reorganization of cortical microtubules in living plant cells
Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin:: Time-sequence observations of the reorganization of cortical microtubules in living plant cells
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DOI：
10.1093/pcp/pce091
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发表时间：
2001-07-01
影响因子：
4.9
通讯作者：
Hasezawa, S
中科院分区：
文献类型：
--
作者：
Kumagai, F;Yoneda, A;Hasezawa, S
在改良的Linsmaier和Skoog培养基中传代培养稳定表达a（G）under bar FP（绿色荧光蛋白）-（t）under bar小泡蛋白融合蛋白（BY-GT 16）的转基因（BY）under bar-2细胞。用Aphidicolin对BY-GT 16细胞进行同步化处理，并用共聚焦激光扫描显微镜（CLSM）观察其微管的动态变化，研究了微管在M/G（1）界面的重组方式。皮层MT最初组织在核周区域，然后它们延伸到细胞皮层，在那里形成亮点。随后，第一皮质MT从斑点迅速伸长，并且它们平行于长轴朝向细胞的远端定向。当平行MT的尖端到达远端时，在分裂部位附近的皮质中随后形成横向皮质MT，如我们先前所建议的[Hasezawa和Nagata（1991）Bet.第104章：206，Nagata等，（1994）Planta 193：567]，在独立的观察中证实，在每个细胞中，平行MT的出现之后是横向MT的出现。我们发现，横向的MT在20-30分钟内遍布整个细胞皮层，而平行的MT消失。这些意见的意义上的模式皮层MT组织进行了讨论。
Transgenic (BY) under bar -2 cells stably expressing a (G) under bar FP (green fluorescent protein)-(t) under bar ubulin fusion protein (BY-GT16) were subcultured in a modified Linsmaier and Skoog medium. The BY-GT16 cells could be synchronized by aphidicolin and the dynamics of their microtubules (MTs) were monitored by the confocal laser scanning microscopy (CLSM), We have succeeded in investigating the mode of reorganization of cortical MTs at the M/G(1) interface. The cortical MTs were initially organized in the perinuclear regions and then they elongated to reach the cell cortex, forming the bright spots there. Subsequently, the first cortical MTs rapidly elongated from the spots and they were oriented parallel to the long axis towards the distal end of the cells. Around the time when the tips of the parallel MTs reached the distal end, the formation of transverse cortical MTs followed in the cortex near the division site, as we had previously suggested [Hasezawa and Nagata (1991) Bet. Acta 104: 206, Nagata et al, (1994) Planta 193: 567], It was confirmed in independent observations that the appearance of the parallel MTs was followed by the appearance of the transverse MTs in each cell. We found that the transverse MTs spread through the whole cell cortex within about 20-30 min, while the parallel MTs disappeared. The significance of these observations on the mode of cortical MT organization is discussed.