Identification of new altered genes in rat cochleae with noise-induced hearing loss
Identification of new altered genes in rat cochleae with noise-induced hearing loss
复制标题
识别噪声引起的听力损失的大鼠耳蜗中新改变的基因。
DOI：
10.1016/j.gene.2012.02.042
复制
发表时间：
2012-05-15
期刊：
影响因子：
3.5
通讯作者：
Qiu, Jianhua
中科院分区：
文献类型：
--
作者：
Han, Yu;Hong, Liu;Qiu, Jianhua
由于噪声应激后在耳蜗中高表达的基因可能对听力具有至关重要的调节作用，因此这些基因的鉴定可能有助于恢复正常的听觉功能。本研究通过使用微阵列和实时聚合酶链反应 (qPCR) 评估大鼠停止噪声暴露 1 小时后基因表达的变化。此外，还观察到听觉阈值的变化和毛细胞的形态变化。这项研究表明，施加的噪音会导致外毛细胞损失和 40-50 分贝的听力损失。总共有 239 个改变的基因涉及免疫系统过程、对压力的反应或对刺激的反应。通过 qPCR 证实了五个上调基因（Reg3b、Lcn2、Serpina3n、Nob1 和 Hamp）的表达。未来的实验将集中于其中几个新的候选基因，并可能提供对潜在听觉病理生理学的深入了解。 (C) 2012 Elsevier B.V. 保留所有权利。
Because genes that are highly expressed in the cochlea after noise stress may have crucial regulatory roles in hearing, the identification of these genes may be useful for restoring normal auditory function. This study assessed altered gene expression at 1 h following the cessation of noise exposure by using microarrays and real-time polymerase chain reaction (qPCR) in rats. In addition, the auditory threshold shifts and morphological changes of hair cells were observed. This study indicated that applied noise induced outer hair cell loss and a 40-50 dB hearing loss. Totally 239 altered genes were involved in the immune system process, response to stress, or response to stimulus. The expression of five up-regulated genes (Reg3b, Lcn2, Serpina3n, Nob1 and Hamp) was confirmed by qPCR. Future experiments will focus on several of these new candidate genes and may provide insight into the underlying auditory pathophysiology. (C) 2012 Elsevier B.V. All rights reserved.