Activation and Alteration of Base Selectivity by Metal Cations in the Functionality-Transfer Reaction for RNA Modification
Activation and Alteration of Base Selectivity by Metal Cations in the Functionality-Transfer Reaction for RNA Modification
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DOI：
10.1021/bc100131j
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发表时间：
2010-08-01
影响因子：
4.7
通讯作者：
Sasaki, Shigeki
中科院分区：
文献类型：
--
作者：
Onizuka, Kazumitsu;Taniguchi, Yosuke;Sasaki, Shigeki
以前，我们报告说，2-亚甲基-1，3-二酮单元的6-硫代鸟苷转移选择性的氨基胞嘧啶在pH 7.0和它的选择性改变为鸟嘌呤碱在pH 9.6。在这项研究中，人们发现，功能转移反应增强的鸟嘌呤碱的选择性，在二价过渡金属阳离子，如Ni 2+和Co2+的存在下，在pH 7.4。
Previously, we reported that the 2-methylidene-1,3-diketone unit of 6-thioguanosine transferred selectively to the amino group of cytosine at pH 7.0 and that its selectivity was changed to the guanine base at pH 9.6. In this study, it was found that the functionality-transfer reaction enhanced selectivity for the guanine base in the presence of divalent transition metal cations such as Ni2+ and Co2+ at pH 7.4.