Accurate staging of reproduction development in Cadenza wheat by non-destructive spike analysis
Accurate staging of reproduction development in Cadenza wheat by non-destructive spike analysis
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DOI：
10.1093/jxb/eraa156
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发表时间：
2020-06-22
影响因子：
6.9
通讯作者：
Wilson, Zoe A.
中科院分区：
文献类型：
--
作者：
Fernandez-Gomez, Jose;Talle, Behzad;Wilson, Zoe A.
小麦是世界上最重要的农作物之一，然而，气候条件变化导致的遗传变异和非生物胁迫的丧失威胁着未来的生产力。繁殖对小麦产量至关重要；然而，花粉发育是对热、冷或干旱等逆境最敏感的发育阶段。需要更好地了解花药和花粉发育是如何调控的，以帮助生产更具弹性的作物，并确保未来的产量增加。然而，在小麦、大麦和水稻等谷物中，花在发育中的假茎中形成，因此准确地确定花物质的分期是非常具有挑战性的。这使得对花的发育进行详细的表型和分子分析非常困难，特别是在植物材料有限的情况下，例如突变或转基因品系。在这里，我们提出了一种准确的方法来克服这个问题，通过对广泛使用的春小麦研究品种卡登扎的生殖发育进行非破坏性的分期。这使用了一个双尺度系统，由此可以根据假茎中的穗大小和穗的位置来预测花药和花粉的发育。该系统提供了一种简单、重复性好的方法，便于对花卉材料进行准确的采样和分析，从而使花药和花粉发育研究成为可能。
Wheat is one of the most important crops in the world; however, loss of genetic variability and abiotic stress caused by variable climatic conditions threaten future productivity. Reproduction is critical for wheat yield; however, pollen development is amongst the developmental stages most sensitive to stresses such as heat, cold, or drought. A better understanding of how anther and pollen development is regulated is needed to help produce more resilient crops and ensure future yield increases. However, in cereals such as wheat, barley, and rice, flowers form within the developing pseudostem and therefore accurate staging of floral materials is extremely challenging. This makes detailed phenotypic and molecular analysis of floral development very difficult, particularly when limited plant material is available, for example with mutant or transgenic lines. Here we present an accurate approach to overcome this problem, by non-destructive staging of reproduction development in Cadenza, the widely used spring wheat research variety. This uses a double-scale system whereby anther and pollen development can be predicted in relation to spike size and spike position within the pseudostem. This system provides an easy, reproducible method that facilitates accurate sampling and analysis of floral materials, to enable anther and pollen developmental research.