Possible involvement of enolase in fluoride resistance in Streptococcus mutans
Possible involvement of enolase in fluoride resistance in Streptococcus mutans
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DOI：
10.1016/j.pdj.2013.10.002
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发表时间：
2014-04-01
影响因子：
0.8
通讯作者：
Kozai, Katsuyuki
中科院分区：
文献类型：
--
作者：
Mitsuhata, Chieko;Puteri, Mega Moeharyono;Kozai, Katsuyuki
变形链球菌是引起龋齿的主要致病菌，经常使用氟化物预防龋齿。然而，尽管常规使用氟化物，儿童仍有龋齿。这可能是由于耐氟变链菌的存在。众所周知，烯醇化酶对氟化物非常敏感，氟化物会抑制变形链球菌的生长。我们研究了功能烯醇化酶在不同菌株中的表达如何影响变形链球菌的耐氟性。我们选择了四种不同的变形链球菌，包括已知的耐氟菌株NCH105。用不同浓度的氟化钠(NaF)对4株菌株进行生长抑制试验。用通透性细胞测定了其烯醇酶活性。对不同菌株的烯醇化酶基因序列进行了比较。UA130和NCH105对氟诱导的生长抑制的抗性强于其他菌株。对通透性细胞的结果表明，在用NaF生长的培养物中，烯醇酶活性的降低并不总是与变形链球菌的生长减少有关。基因分析表明，UA130和NCH105均存在烯醇化酶点突变。版权所有(C)2013日本儿科牙科学会。爱思唯尔有限公司出版。保留所有权利。
Streptococcus mutans is the principal causative agent of dental caries, for which fluoride is often used for prevention. However, despite routine use of fluoride, children continue to have tooth decay. This may be because of the presence of fluoride-resistant S. mutans. It is known that the enzyme enolase is very sensitive to fluoride, which would decrease S. mutans growth. We investigated how the expression of functional enolase in different strains of S. mutans affects fluoride resistance. We selected four different S. mutans strains, including NCH105, which is known to be fluoride-resistant. The four strains were checked for growth inhibition by different sodium fluoride (NaF) concentrations. Enolase activity was also investigated using permeabilized cells. Genetic sequences for enolase were compared across the strains. UA130 and NCH105 were more resistant to fluoride- induced growth inhibition than the other strains. Results for permeabilized cells indicate that decreased enolase activity was not always associated with decreased S. mutans growth in cultures grown with NaF. Gene analysis showed that UA130 and NCH105 both had enolase point mutations. Copyright (C) 2013 The Japanese Society of Pediatric Dentistry. Published by Elsevier Ltd. All rights reserved.