Meiotic Prophase Requires Proteolysis of M Phase Regulators Mediated by the Meiosis-Specific APC/CAma1
Meiotic Prophase Requires Proteolysis of M Phase Regulators Mediated by the Meiosis-Specific APC/CAma1
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DOI：
10.1016/j.cell.2012.08.044
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发表时间：
2012-10-26
期刊：
影响因子：
64.5
通讯作者：
Zachariae, Wolfgang
中科院分区：
文献类型：
--
作者：
Okaz, Elwy;Argueello-Miranda, Orlando;Zachariae, Wolfgang
尽管增殖细胞在DNA复制后不久进入M期，但减数分裂的第一个M期之前是一个延长的前期，其中同源染色体进行重组。前期I的退出由重组检查点（RC）控制，在酵母中，该检查点抑制B型细胞周期蛋白和其他M期调节因子表达所需的减数分裂特异性转录因子Ndt 80。我们表明，延长前期I还需要抑制潜伏的，有丝分裂细胞周期控制的后期促进复合物（APC/C）和其减数分裂特异性激活剂Ama 1，这触发了M期调节剂和Ndd 1，有丝分裂转录因子的亚基的降解。ama 1 Delta突变体过早地从前期I退出，并且独立于RC，这导致重组缺陷和染色体错误分离。因此，减数分裂机制对前期I的控制依赖于减数分裂特异性形式的APC/C对替代的有丝分裂机制的抑制。
Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1 Delta mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.