Nippostrongylus brasiliensis: Cytokine responses and nematode expulsion in normal and IL-4-deficient mice
Nippostrongylus brasiliensis: Cytokine responses and nematode expulsion in normal and IL-4-deficient mice
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DOI：
10.1006/expr.1996.0090
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发表时间：
1996-10-01
影响因子：
2.1
通讯作者：
Maizels, RM
中科院分区：
文献类型：
--
作者：
Lawrence, RA;Gray, CA;Maizels, RM
感染这种线虫会导致以Th2为主的免疫反应。我们描述了这种反应在局部和系统环境中的动态。Th2型反应首先出现在肠系膜淋巴结，寄生虫抗原特异性增殖和IL-4/IL-5的释放在感染后7-9天达到高峰，不久成虫就会从肠道中排出。肠系膜淋巴结未见干扰素-γ反应。脾的反应一般比肠系膜淋巴结的反应晚2-3天，并且表现出更大程度的Th1型细胞因子的产生。结果表明，巴西新月浑圆线虫是一种强有力的IL-4诱导剂，只有6只感染的巴西新月浑圆线虫幼虫能在肠系膜淋巴结处产生IL-4，但只有高剂量的幼虫(600头)才能在脾中产生IL-4。感染后用ConA或寄生虫抗原刺激的淋巴结细胞产生IL-4的水平相似，表明这种寄生虫对多克隆Th2的刺激程度。对IL-4缺陷小鼠的感染表明，尽管没有依赖IL-4的Th2反应，这些小鼠能够减少产卵并在与完全具有免疫能力的动物相似的时间框架内驱逐成虫。这些结果强调了Th2对巴西新城疫免疫应答的大小，也表明IL-4不是发展对巴西新城疫免疫的先决条件。(C)1996年学术出版社。
Infection with the nematode, Nippostrongylus brasiliensis, results in a Th2-dominated immune response. We describe the dynamics of this response in both local and systemic environments. Th2-type responses were evident first in the mesenteric lymph node, with parasite antigen-specific proliferation and IL-4/IL-5 release peaking at Days 7-9 postinfection, shortly before expulsion of the adult worms from the gut. IFN-gamma responses were not observed in the mesenteric lymph node. Responses in the spleen generally followed those in the mesenteric lymph nodes by 2-3 days and showed a greater degree of Th1-type cytokine production. N. brasiliensis was shown to be a powerful inducer of IL-4 responses with as few as six infective N. brasiliensis larvae eliciting IL-4 production in the mesenteric lymph node, but only high doses of larvae (600) elicited IL-4 secretion in the spleen. Similar levels of IL-4 production by lymph node cells stimulated with Con A or parasite antigen postinfection indicated the extent of polyclonal Th2 stimulation by this parasite. Infection of IL-4-deficient mice showed that despite the absence of IL-4-dependent Th2 responses, these mice were able to curtail egg production and expel adult N. brasiliensis in a time frame similar to that of fully immunocompetent animals. These results emphasize the magnitude of the Th2 response to N. brasiliensis and also show that IL-4 is not a prerequisite for the development of immunity to N. brasiliensis. (C) 1996 Academic Press, Inc.