Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting
Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting
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DOI：
10.1007/s10577-006-1083-0
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发表时间：
2006-11
影响因子：
2.6
通讯作者：
H. Zhu;D. Ma;J. Gui
中科院分区：
文献类型：
--
作者：
H. Zhu;D. Ma;J. Gui
以异育银鲫（Carassius auratus gibelio）和鲫鱼（Carassius auratus）为研究对象，分别对162条染色体和100条染色体的异育银鲫和鲫鱼的5S rDNA进行了分离和序列测定，并制备了染色体定位荧光探针，以探讨异育银鲫和鲫鱼的倍性起源和进化关系。荧光原位杂交（FISH）结果表明，银鲫的5SrDNA信号主要位于3条亚端着丝粒染色体的短臂上，而鲫鱼的5SrDNA信号主要位于2条亚端着丝粒染色体的短臂上。此外，在两个物种的其他染色体上检测到一些小的信号。同时，用玻璃针显微分离异育银鲫中期染色体，并采用简并寡核苷酸引物-聚合酶链反应（DOP-PCR）对分离的染色体进行体外扩增。值得注意的是，当DOP-PCR产生的探针制备从每个单染色体杂交，三个相同大小的染色体被涂在每个银鲫中期，而只有两个染色的染色体被观察到在每个鲫鱼中期蔓延。与二倍体鲫鲤相比，异育银鲫是三倍体起源。
5S ribosomal DNA (rDNA) was isolated and sequenced from the gibel carpCarassius auratus gibeliowith 162 chromosomes and crucian carpCarassius auratuswith 100 chromosomes, and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species. Using fluorescencein-situhybridization (FISH), major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp. In addition, some minor signals were detected on other chromosomes of both species. Simultaneously, six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles, and the isolated chromosomes were amplifiedin vitroby degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). Significantly, when the DOP-PCR-generated probes prepared from each single chromosome were hybridized, three same-sized chromosomes were painted in each gibel carp metaphase, whereas only two painted chromosomes were observed in each crucian carp metaphase spread. The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp.