Effect of compression milling on cellulose structure and on enzymatic hydrolysis kinetics
Effect of compression milling on cellulose structure and on enzymatic hydrolysis kinetics
复制标题
压缩研磨对纤维素结构和酶水解动力学的影响
DOI：
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发表时间：
1982
影响因子：
3.8
通讯作者：
Charles Macy
中科院分区：
文献类型：
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作者：
D. Ryu;Sun Bok Lee;Thomas H. Tassinari;Charles Macy
研究了纤维素结构通过压缩碾磨的变化，并以结晶度、可及性、比表面积和聚合度表示。实验测定了反应动力学参数、最大反应速率和米氏常数。基于实验结果，提出了一个两相模型，该模型基于纤维素酶复合物对结晶相和非晶相的同时作用而降解纤维素。并将纤维素可及性与动力学参数的关系与模型预测值进行了比较。发现了很好的一致性，尽管两相假设是纤维素中真实有序状态的简化。
The changes in the cellulose structure by compression milling were studied and expressed in terms of crystallinity, accessibility, specific surface area, and degree of polymerization. The kinetic parameters, maximum reaction rate, and Michaelis constant were determined experimentally. Based on the experimental results a two‐phase model, which is based on the degradation of cellulose by simultaneous actions of the cellulase complex on the crystalline and amorphous phases, is proposed. The relationships between cellulose accessibility and the kinetic parameters were compared with those predicted by the model. A good agreement was found, although the two‐phase hypothesis is a simplification of the true state of order in cellulose.