Evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Bruker Biotyper for identification of Penicillium marneffei, Paecilomyces species, Fusarium solani, Rhizopus species, and Pseudallescheria boydii
Evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Bruker Biotyper for identification of Penicillium marneffei, Paecilomyces species, Fusarium solani, Rhizopus species, and Pseudallescheria boydii
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DOI：
10.3389/fmicb.2015.00679
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发表时间：
2015-07-08
影响因子：
5.2
通讯作者：
Hsueh, Po-Ren
中科院分区：
文献类型：
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作者：
Chen, Ying-Sheng;Liu, Yen-Hung;Hsueh, Po-Ren
我们评估了基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF MS)、MALDI Bruker Biotyper 系统（microflex LT；Bruker Daltonik GmbH，不来梅，德国）对 50 种临床遇到的霉菌分离株的鉴定性能，包括马尔尼菲青霉 (n = 28)、拟青霉属 (n = 12)、茄病镰刀菌(n = 6)、根霉属 (n = 3) 和 Pseudallescheria boydii (n = 1)。通过使用引物 ITS1 和 ITS4 对内部转录间隔区 (ITS) 区域进行序列分析，将分离株鉴定到物种水平。通过 MALDI-TOF MS，28 个遗传特征良好的马尔尼菲疟原虫分离株均未鉴定为马尔尼菲疟原虫，因为布鲁克通用文库 (DB 5627) 或布鲁克丝状真菌文库 V1.0 中均不存在马尔尼菲疟原虫。然而，根据 MALDI Biotyper 系统对一种马尔尼菲疟原虫菌株 (NTUH-3370) 新创建的数据库，准确鉴定为马尔尼菲疟原虫 (分值 >= 2.000) 的率为 85.7%。对这 22 种非马尔尼菲霉菌分离株的测序分析显示，有 7 种拟青霉、6 种茄镰孢、4 种淡紫拟青霉，以及中华拟青霉、无根根霉、米根霉、小孢子霉和波氏拟青霉各 1 种。尽管所有 7 个 P variotii 分离株、6 个 F. solani 中的 4 个、4 个 P lilacinus 中的 2 个、根霉菌属 3 个分离株中的 2 个以及 P boydii 分离株在 MALDI-TOF MS 和测序分析中的鉴定结果一致，但这些分离株的分值均是
We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), the MALDI Bruker Biotyper system (microflex LT; Bruker Daltonik GmbH, Bremen, Germany), on the identification of 50 isolates of clinically encountered molds, including Penicillium marneffei (n = 28), Paecilomyces species (n = 12), Fusarium solani (n = 6), Rhizopus species (n = 3), and Pseudallescheria boydii (n = 1). The isolates were identified to species levels by sequence analysis of the internal transcribed spacer (ITS) regions using primers ITS1 and ITS4. None of the 28 genetically well characterized isolates of P marneffei were identified as P marneffei by MALDI-TOF MS, because P marneffei was not present in either Bruker general library (DB 5627) or Bruker filamentous fungi library V1.0. However, the rate of accurate identification as P marneffei (score value >= 2.000) was 85.7% based on newly created database from one P marneffei strain (NTUH-3370) by MALDI Biotyper system. Sequencing analysis of these 22 non-P marneffei isolates of molds revealed seven Paecilomyces variotii, six F. solani, four Paecilomyces lilacinus, and one each of Paecilomyces sinensis, Rhizopus arrhizus, R. oryzae, R. microspores, and P boydii. Although all the seven P variotii isolates, four of the six F. solani, two of the four P lilacinus, and two of the three isolates of Rhizopus species, and the P boydii isolate had concordant identification results between MALDI-TOF MS and sequencing analysis, the score values of these isolates were all of