Pseudorabies virus propagated in rabbit kidney-derived RK13 cells is neutralized by natural IgM antibodies in normal swine serum which specifically lyse host cells.
Pseudorabies virus propagated in rabbit kidney-derived RK13 cells is neutralized by natural IgM antibodies in normal swine serum which specifically lyse host cells.
复制标题
DOI：
10.1292/jvms.67.229
复制
发表时间：
2005-03
期刊：
影响因子：
--
通讯作者：
Sunao Hayashi;Y. Takashima;H. Otsuka
中科院分区：
文献类型：
--
作者：
Sunao Hayashi;Y. Takashima;H. Otsuka
用特异性无病原猪血清通过激活补体中和在兔肾源RK-13细胞（PRV-RK）中增殖的伪狂犬病病毒（PRV）。用乙二醇-双氨乙基醚-N，N，N ′，N ′-四乙酸（EGTA）或乙二胺四乙酸（EDTA）处理猪血清后，病毒中和活性丧失。抗C1 q和抗IgM抗体也抑制病毒中和活性。尽管IgG去除的猪血清中和PRV，但IgM和不含IgG的猪血清失去病毒中和活性。猪血清与RK-13细胞（但不与猪肾衍生的CPK细胞）在4 ℃下预孵育消除了对PRV-RK的病毒中和活性。结果表明，猪血清含有针对RK-13细胞表面抗原的天然IgM，并且该表面抗原在出芽过程中整合到PRV包膜中。因此，猪血清中的天然IgM与病毒包膜上的RK-13抗原反应，激活补体级联反应并中和PRV-RK。
Pseudorabies virus (PRV) propagated in rabbit kidney-derived RK-13 cells (PRV-RK) was neutralized by serum obtained from specific pathogen-free pigs through the activation of complement. The virus-neutralizing activity of swine serum was lost after treatment with ethylene glycol-bis-aminoethylether-N,N,N',N'-tetraacetic acid (EGTA) or ethylenediaminetetraacetic acid (EDTA). Anti-C1q and anti-IgM antibodies also inhibited virus-neutralizing activity. Though IgG-depleted swine serum neutralized PRV, IgM and IgG-free swine serum lost virus-neutralizing activity. Pre-incubation of swine serum with RK-13 cells, but not with swine kidney-derived CPK cells, at 4 degrees C eliminated the virus-neutralizing activity to PRV-RK. Results indicated that swine serum contained natural IgM against an antigen(s) on the RK-13 cell surface and that this surface antigen was integrated into the PRV envelope during the budding process. Thus the natural IgM in swine serum reacted with the RK-13 antigen on the viral envelope, activated the complement cascade and neutralized the PRV-RK.