The pH sensing receptor AopalH plays important roles in the nematophagous fungus Arthrobotrys oligospora
The pH sensing receptor AopalH plays important roles in the nematophagous fungus Arthrobotrys oligospora
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pH 传感受体 AopalH 在食线虫少孢节孢菌中发挥重要作用
DOI：
10.1016/j.funbio.2019.05.008
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发表时间：
2019-07-01
期刊：
影响因子：
2.5
通讯作者：
Zhang, Ke-Qin
中科院分区：
文献类型：
--
作者：
Li, Juan;Wu, Runian;Zhang, Ke-Qin
子囊菌中存在高度保守的PacC/Rim 101信号通路，以介导环境pH传感。对于致病真菌，这一途径不仅使真菌能够在较宽的pH范围内生长，而且还决定了这些真菌是否能够成功地定殖和入侵目标宿主。在pal/PacC通路内，palH是具有七跨膜结构域的推定环境pH传感器。为了表征嗜食真菌Arthrobotelioligospora中palH同源物AopalH的功能，我们通过同源重组敲除了AopalH的编码基因，转化体表现出更慢的生长速率，对阳离子和过氧化胁迫更敏感，以及减少分生孢子和减少陷阱形成，这表明pH调节系统在嗜食真菌中具有关键功能。我们的研究结果提供了新的见解真菌的pH响应和调节的机制。(C)2019年英国真菌学会由爱思唯尔有限公司出版。保留所有权利。
There is well-conserved PacC/Rim101 signaling among ascomycete fungi to mediate environmental pH sensing. For pathogenic fungi, this pathway not only enables fungi to grow over a wide pH range, but it also determines whether these fungi can successfully colonize and invade the targeted host. Within the pal/PacC pathway, palH is a putative ambient pH sensor with a seven-transmembrane domain. To characterize the function of a palH homolog, AopalH, in the nematophagous fungus Arthrobotrys oligospora, we knocked out the encoding gene of AopalH through homologous recombination, and the transformants exhibited slower growth rates, greater sensitivities to cationic and hyperoxidation stresses, as well as reduced conidiation and reduced trap formation, suggesting that the pH regulatory system has critical functions in nematophagous fungi. Our results provide novel insights into the mechanisms of pH response and regulation in fungi. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.