AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis
AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis
复制标题
AC002454.1和CDK6协同促进子宫内膜异位症中子宫内膜细胞迁移和侵袭
DOI：
10.1530/rep-19-0005
复制
发表时间：
2019-06-01
期刊：
影响因子：
3.8
通讯作者：
Wang, Danbo
中科院分区：
文献类型：
--
作者：
Liu, Jing;Wang, Yang;Wang, Danbo
前期lncRNA微阵列筛选发现，AC002454.1基因在子宫内膜异位症（EMS）中高表达，且这些表达水平与细胞周期蛋白依赖性激酶6（CDK 6）高度相关。本研究探讨了AC002454.1和CDK 6在子宫内膜组织中的表达水平及其相关性，以及这些表达变化对在位子宫内膜细胞生物学行为的影响。我们证实AC002454.1和CDK 6 mRNA和蛋白在EMS患者的异位和在位子宫内膜组织中高度表达，并且明显相关。AC002454.1和CDK 6在体外均能正向调节在位子宫内膜细胞的增殖、迁移和侵袭能力，促进细胞由G 0/G1期向S期转化。AC002454.1和CDK 6可能具有协同作用，从而影响子宫内膜细胞的生物学行为，进而促进EMS的进展。
Previous lncRNA microarray screening found that the AC002454.1 gene was highly expressed in endometriosis (EMS), and these expression levels were highly correlated with cyclin-dependent kinase-6 (CDK6). This study investigated the expression level and correlation between AC002454.1 and CDK6 in endometrium tissues and the influence of these changes in expression upon the biological behavior of eutopic endometrial cells. We confirmed AC002454.1 and CDK6 mRNA and protein were highly expressed in ectopic and eutopic endometrial tissue from patients with EMS and were clearly correlated. In vitro, both AC002454.1 and CDK6 positively regulated the proliferation, migration and invasion ability of eutopic endometrial cells and could promote the transformation of cells from G0/G1 phase to S phase. AC002454.1 and CDK6 may have synergistic effects, thereby affecting the biological behavior of endometrial cells, and thus promote the progression of EMS.