IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOGENOUS NERVE GROWTH-FACTOR
IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOGENOUS NERVE GROWTH-FACTOR
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DOI：
10.1038/312364a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
RUSH, RA
中科院分区：
文献类型：
--
作者：
RUSH, RA
神经生长因子(NGF)被认为是交感神经和初级感觉神经元发育所必需的营养分子。在新生小鼠和大鼠中，注射神经生长因子会导致存活神经元的数量增加，而注射NGF抗血清则会减少神经元的存活。有人认为，该因子是由相关靶组织产生和分泌的，为内生神经提供营养支持。神经生长因子的合成部位尚不清楚；在知道产生神经生长因子的细胞类型之前，无法确定该分子的确切生理作用。免疫组织化学用于定位内源性NGF在大鼠虹膜组织中的位置，在该组织中有充分的生化证据表明NCG活性的产生。神经生长因子在失交感神经支配或培养的虹膜平滑肌细胞中很容易检测到，但在雪旺细胞中检测不到。
Nerve growth factor (NGF) has been proposed as a trophic molecule essential for the development of sympathetic and primary sensory neuron. In newborn mice and rats, administration of nerve growth factor results in an increase in the number of surviving neurons, whereas administration of antiserum to NGF decreases neuronal survival. It was proposed that the factor is produced and secreted by the relevant target tissues to provide trophic support for the ingrowing nerves. The site of synthesis of nerve growth factor is still unknown; a precise physiological role for the molecule cannot be ascribed until the cell types that produce it are known. Immunohistochemistry was used to localize endogenous NGF in the rat iris, a tissue in which there is sound biochemical evidence for the production of NCG activity. NGF was readily detected in Schwann cells, but not in smooth muscle cells of the iris when it is sympathetically denervated or cultured.