Cell-free fat extract promotes tissue regeneration in a tissue expansion model
Cell-free fat extract promotes tissue regeneration in a tissue expansion model
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DOI：
10.1186/s13287-020-1564-7
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发表时间：
2020-02-04
影响因子：
7.5
通讯作者：
Zhang, Wen Jie
中科院分区：
文献类型：
--
作者：
Deng, Mingwu;Wang, Xiangsheng;Zhang, Wen Jie
背景组织扩张术在整形外科中发挥着重要作用。如何提高扩张皮肤的质量，缩短扩张周期，仍是值得研究的问题。我们先前的研究发现，无细胞脂肪提取物(CEFFE)具有促血管生成和促增殖活性。然而，CEFFE在组织扩张中的作用尚不清楚。本研究的目的是评价CEFFE对组织扩张的影响。方法采用大鼠组织扩张法。动物皮下注射CEFFE。组织扩张4周后，观察皮肤坏死率和回缩率，组织学检测真皮和表皮厚度，抗CD31染色检测血管密度，增殖细胞核抗原染色检测细胞增殖，Western印迹分析特异性蛋白表达。此外，还观察了CEFFE对体外培养的HaCaT细胞增殖和细胞周期的影响。结果CEFFE治疗可明显降低扩张皮肤的坏死率和回缩。与未经处理的皮肤相比，经CEFFE处理的皮肤的表皮和真皮层厚度较高。经CEFFE处理后，扩张皮肤的表皮血管密度增加，细胞增殖增加。此外，CEFFE还能显著增加HaCaT细胞血管内皮生长因子受体、表皮生长因子受体、1型和3型胶原的表达，并促进HaCaT细胞的增殖。结论CEFFE通过促进血管生成和细胞增殖，改善扩张皮肤的质量。它有可能在临床上用于扩大组织扩张。
Background Tissue expansion techniques play an important role in plastic surgery. How to improve the quality of the expanded skin and shorten the expansion period are still worth investigating. Our previous studies found that a cell-free fat extract (CEFFE) possessed pro-angiogenic and pro-proliferative activities. However, the role of CEFFE on tissue expansion has remained unclear. The purpose of this study was to evaluate the effect of CEFFE on tissue expansion. Methods A rat tissue expansion model was used. Animals were treated with CEFFE by subcutaneous injection. After 4 weeks of tissue expansion, the skin necrosis and retraction rates were evaluated, the thicknesses of the epidermis and dermis were determined by histological analyses, blood vessel density was measured by anti-CD31 staining, cell proliferation was assessed by proliferating cell nuclear antigen staining, and the expression of specific proteins was evaluated by western blot analyses. In addition, the effects of CEFFE on the proliferation and cell cycle of cultured HaCaT cells were evaluated in vitro. Results CEFFE treatment significantly decreased the necrosis rate and retraction of the expanded skin. The thickness of the epidermal and dermal layers was higher in CEFFE-treated compared to untreated skin. The density of blood vessels and cell proliferation in the epidermis of the expanded skin was improved by CEFFE treatment. In addition, CEFFE treatment significantly increased the expression of the vascular endothelial growth factor receptor, epidermal growth factor receptor, collagen type 1, and collagen type 3. CEFFE also increased the proliferation of HaCaT cells in culture. Conclusions CEFFE improves the quality of the expanded skin by promoting angiogenesis and cell proliferation. It could be potentially used clinically for augmenting tissue expansion.