The DNA cleavage and packaging protein encoded by the UL33 gene of herpes simplex virus 1 associates with capsids
The DNA cleavage and packaging protein encoded by the UL33 gene of herpes simplex virus 1 associates with capsids
复制标题
DOI：
10.1016/j.virol.2004.03.044
复制
发表时间：
2004-07-01
期刊：
影响因子：
3.7
通讯作者：
Baines, JD
中科院分区：
文献类型：
--
作者：
Beard, PM;Baines, JD
单纯疱疹病毒1型U(L)33基因编码一种蛋白质(PU(L)33)，该蛋白对链状疱疹病毒DNA的切割和包装形成预先形成的衣壳是必不可少的。先前的数据表明，U(L)33蛋白与已知与衣壳相关的U(L)15和U(L)28编码的切割和包装蛋白相互作用。对来自流产包装事件的缺乏DNA的纯化A衣壳、缺乏DNA的B衣壳和含有DNA的C衣壳的检测显示，U(L)33蛋白与所有三种衣壳类型都有关。A衣壳中U(L)33蛋白含量高于B衣壳。衣壳结合对盐酸胍敏感，不受U(L)15或U(L)28的影响。PU(L)33的衣壳结合也独立于U(L)6，U(L)6被认为编码DNA插入的入口。这些数据表明，PU(L)33可能是衣壳相关分子机制的一部分，该机制将裂解的基因组DNA转移到衣壳内部。(C)2004 Elsevier Inc.保留所有权利。
The U(L)33 gene of herpes simplex virus 1 (HSV-1) encodes a protein (pU(L)33) that is essential for the cleavage and packaging of concatameric herpesvirus DNA into preformed capsids. Previous data have suggested that the U(L)33 protein interacts with the cleavage and packaging proteins encoded by U(L)15 and U(L)28 that are known to associate with capsids. Examination of purified A capsids that lack DNA and are derived from aborted packaging events, B capsids that lack DNA, and C capsids that contain DNA revealed an association of the U(L)33 protein with all three capsid types. More U(L)33 protein was detected in A capsids than was present in B capsids. Capsid association was susceptible to guanidine-HCl treatment and independent of the presence of U(L)15 or U(L)28. Capsid association of pU(L)33 was also independent of U(L)6, which is believed to encode the portal into which DNA is inserted. These data suggest that pU(L)33 may act as part of the capsid-associated molecular machinery that translocates cleaved genomic DNA into the capsid interior. (C) 2004 Elsevier Inc. All rights reserved.