Immunohistochemical localization of superoxide dismutase in the human ovary.
Immunohistochemical localization of superoxide dismutase in the human ovary.
复制标题
人卵巢中超氧化物歧化酶的免疫组织化学定位。
作者：
Masahide Shiotani;Yoichi Noda;K. Narimoto;Kimitoshi Imai;Mori Takahide;K. Fujimoto;Kazuo Ogawa
为了阐明超氧化物歧化酶(SOD)在卵巢中的生理功能，我们用免疫组织化学方法研究了CuZn-SOD在人卵巢中的分布。用酶联免疫吸附测定法测定卵泡液中铜锌超氧化物歧化酶的含量。生发上皮和白膜无或弱免疫反应。在无腔卵泡中也未见或有弱染色。一旦卵泡开始形成窦腔，内膜细胞就开始显示出强烈的SOD免疫染色，而颗粒细胞和外膜细胞则不表达。在妊娠黄体中，卵泡膜细胞和黄体颗粒细胞分别呈浓染和中度染色。排卵前卵泡液中铜锌超氧化物歧化酶含量为0.222+/-0.186 ng/mg蛋白(平均值+/-SD)。本研究首次证实了超氧化物歧化酶在人卵巢中的免疫组织化学分布。考虑到超氧化物歧化酶催化超氧阴离子自由基的歧化反应，本研究结果提示内膜细胞在卵泡成熟过程中作为血滤泡屏障在保护发育中的卵母细胞免受氧自由基的伤害中起重要作用。
To elucidate the physiological function of superoxide dismutase (SOD) in the ovary, we examined the immunohistochemical distribution of CuZn-SOD in the human ovary. We also measured the CuZn-SOD concentration in human follicular fluid by enzyme-linked immunosorbent assay (ELISA). The germinal epithelium and tunica albuginea showed no or weak immunoreactivity. No or weak staining activity was also observed in the non-antral follicle. Once the follicle began to form the antral cavity, theca interna cells began to show intensive immunostaining of SOD, as compared with no staining in the granulosa and theca externa cells. In the gestational corpus luteum, theca and granulosa lutein cells showed intensive and moderate staining activity, respectively. The concentration of CuZn-SOD was 0.222 +/- 0.186 ng/mg protein (mean +/- SD) in the preovulatory follicular fluid. In the present study, the immunohistochemical distribution of SOD was confirmed in the human ovary for the first time. Taking into consideration the fact that SOD catalyses the dismutation reaction of superoxide anion radicals, the present results suggest that theca interna cells play an important role in the protection of the developing oocyte from oxygen radicals by acting as a blood-follicular barrier during follicle maturation.