Moringa Oleifera Oil Modulates Rumen Microflora to Mediate In Vitro Fermentation Kinetics and Methanogenesis in Total Mix Rations
Moringa Oleifera Oil Modulates Rumen Microflora to Mediate In Vitro Fermentation Kinetics and Methanogenesis in Total Mix Rations
复制标题
辣木油调节瘤胃小孔以介导总混合日粮中的体外发酵动力学和产甲烷作用
DOI：
10.1007/s00284-020-01935-2
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发表时间：
2020-03-04
影响因子：
2.6
通讯作者：
Yang Chengjian
中科院分区：
文献类型：
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作者：
Ebeid, Hossam M.;Li Mengwei;Yang Chengjian
本研究旨在评价辣木籽油（MOSO）对不同全混合日粮（TMR）下瘤胃微生物区系的调节作用。以3种不同粗精比的TMR（R70：C30、R50：C50和R30：C70）为底物，采用分批培养技术进行体外发酵研究。结果表明，在不同粗精比的日粮中添加MOSO显著（P < 0.05）改变了CH 4的产生和发酵参数。M. 3%和4%的油茶籽油显著提高了R70：C30全混合日粮中微生物蛋白（MCP）和丙酸盐的含量（P < 0.01），但降低了乙酸盐的含量。两个水平的MOSO（3%和4%）二次增加（P = 0.001）MCP和减少乙酸在R30：C70，而所有水平增加丙酸。对于R30：C70和R50：C50日粮，MOSO线性和二次曲线降低（P < 0.001）原生动物和产甲烷菌的数量;然而，原生动物，产甲烷菌和细菌在R70：C30日粮中显著增加，而真菌数量没有任何变化。在高粗饲料日粮（R70：C30）中添加较高水平的MOSO（4%）对细菌物种多样性（Shannon指数）和均匀性产生负面影响。此外，低水平添加对高粗饲料中厚壁菌门/拟杆菌门比值的降低更为明显。辣木油也刺激普雷沃氏菌在高和低粗饲料饮食，表明其调解瘤胃酸中毒的潜力。总之，MOSO通过有效调节瘤胃微生物组来增强发酵动力学并降低CH 4产生。
This study was conducted to evaluate potential of Moringa oleifera seed oil (MOSO) to modulate rumen microflora to mitigate methane (CH4) production in different total mixed rations (TMRs). Three TMRs with different roughage (R) to concentrate (C) ratio were used as substrates (R70:C30, R50:C50 and R30:C70) for in vitro fermentation study using batch culture technique. Results revealed that supplementation of MOSO in different rations with variable roughage to concentrates ratio altered (P < 0.05) CH4 production and fermentation parameters. M. oleifera seed oil at 3 and 4% increased (P < 0.01) microbial protein (MCP) and propionate concentration for all rations but decreased acetate concentration in R70:C30 TMR. Two levels of MOSO (3 and 4%) quadratically increased (P = 0.001) MCP and decreased acetate in R30:C70 while all levels increased propionate. For R30:C70 and R50:C50 rations, MOSO linearly and quadratically decreased (P < 0.001) protozoa and methanogen counts; however, protozoa, methanogens and bacteria were significantly increased in R70:C30 ration without any change in fungal counts. Supplementation of higher level of MOSO (4%) in high roughage ration (R70:C30) showed negative effects on diversity (Shannon index) and evenness of bacterial species as compared to control and lower oil level. Moreover, it also decreased Firmicutes to Bacteroidetes ratio in high roughage rations more obviously at lower levels. Moringa oil also stimulated Prevotella in both high and low roughages diets that indicates its potential to mediate rumen acidosis. Conclusively, MOSO enhanced fermentation kinetics and decreased CH4 production through effective modulation of rumen microbiome.