DESIGN OF SEQUENCE-SPECIFIC DNA-BINDING MOLECULES
DESIGN OF SEQUENCE-SPECIFIC DNA-BINDING MOLECULES
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DOI：
10.1126/science.2421408
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发表时间：
1986-04-25
期刊：
影响因子：
56.9
通讯作者：
DERVAN, PB
中科院分区：
文献类型：
--
作者：
DERVAN, PB
碱基序列信息可以存储在右手双螺旋DNA （B-DNA）的局部结构中。问题出现了，是否一套规则的三维读出B-DNA螺旋可以开发。这将允许设计结合任何特定序列和位点大小的DNA的合成分子。每个新的合成序列特异性dna结合分子的开发有四个阶段：设计，合成，序列特异性测试和设计的重新评估。这种方法已经产生了双霉素福马酰胺，这是一种合成的新月形寡肽，在双螺旋DNA的小凹槽中结合9个相邻的腺嘌呤-胸腺嘧啶碱基对。
Base sequence information can be stored in the local structure of right-handed double-helical DNA (B-DNA). The question arises as to whether a set of rules for the three-dimensional readout of the B-DNA helix can be developed. This would allow the design of synthetic molecules that bind DNA of any specific sequence and site size. There are four stages of development for each new synthetic sequence-specific DNA-binding molecule: design, synthesis, testing for sequence specificity, and reevaluation of the design. This approach has produced bis(distamycin)fumaramide, a synthetic, crescent-shaped oligopeptide that binds nine contiguous adenine-thymine base pairs in the minor groove of double-helical DNA.