Intranasal administration of synthetic recombinant peptide-based vaccine protects mice from infection by Schistosoma mansoni
Intranasal administration of synthetic recombinant peptide-based vaccine protects mice from infection by Schistosoma mansoni
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DOI：
10.1128/iai.67.9.4360-4366.1999
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发表时间：
1999-09-01
影响因子：
3.1
通讯作者：
Arnon, R
中科院分区：
文献类型：
--
作者：
Ben-Yedidia, T;Tarrab-Hazdai, R;Arnon, R
血吸虫病是一种慢性衰弱性疾病，每年造成大量死亡和发病，特别是在热带和亚热带地区。源自曼氏血吸虫保护性表面蛋白 9B-Ag 的表位（称为 9B 肽-1）先前显示，当与牛血清白蛋白缀合并在完全弗氏佐剂中皮下施用时，对小鼠具有保护作用。本工作在沙门氏菌疫苗株的鞭毛蛋白中表达了这种保护肽，并将分离的重组鞭毛用于小鼠的免疫。由于在寄生虫侵入宿主期间，血吸虫首先迁移至肺部，因此采用鼻内给药途径以在感染的早期阶段阻止寄生虫。这种在鞭毛蛋白中表达的肽的鼻内免疫，在不添加佐剂的情况下，产生了显着的体液反应，并且还导致了针对攻击感染的保护，表现为蠕虫负担平均减少了 42%。
Schistosomiasis is the cause of a chronic debilitating disease which accounts for significant mortality and morbidity every year, especially in tropical and subtropical areas. An epitope derived from the protective surface protein 9B-Ag of Schistosoma mansoni, designated 9B peptide-1, was previously show ed to be protective in mice when conjugated to bovine serum albumin and administered subcutaneously in complete Freund's adjuvant. In this work, this protective peptide was expressed in the flagellin of a Salmonella vaccine strain, and the isolated recombinant flagella were used for immunization of mice. Since during the invasion of the parasite into the host the schistosomula migrate first to the lungs, the intranasal route of administration was employed in order to halt the parasite at an early stage of the infection. Such intranasal immunization with this peptide expressed in flagellin, without the addition of adjuvants, resulted in a significant humoral response and also led to protection against challenge infection, manifested as a reduction of the worm burden by an average of 42%.