Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery
Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery
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DOI：
10.1002/term.2012
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发表时间：
2017-04-01
影响因子：
3.3
通讯作者：
Kojima，Hiromi
中科院分区：
文献类型：
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作者：
Hama，Takanori;Yamamoto，Kazuhisa;Kojima，Hiromi
中耳手术后，中耳腔和乳突腔的黏膜再生非常重要。然而，中耳粘膜的上皮化被延迟，因为没有上皮化的慢性炎症破坏了气体交换和临床功能。中耳中的这些环境条件导致新形成的鼓膜的术后回缩和粘连。因此，如果暴露的中耳骨表面的粘膜能够在手术后迅速再生，则可潜在地改善中耳炎和粘连性中耳疾病的手术治疗。在这项研究中，我们成功地产生了一个细胞片，设计用于术后治疗中耳炎。我们使用鼻细胞来创建人工中耳粘膜细胞片，其具有与中耳粘膜相似的三维（3D）构型。该薄片由多层粘膜上皮和下层结缔组织组成，与正常中耳粘膜相似。这一结果表明，组织工程化粘膜细胞片将有助于减少中耳手术后的并发症，并有望在未来的临床应用中发挥作用。版权所有© 2015约翰威利父子有限公司.
Postoperative mucosal regeneration of the middle ear cavity and the mastoid cavity is of great importance after middle ear surgery. However, the epithelialization of the mucosa in the middle ear is retarded because chronic inflammation without epithelialization aggravates gas exchange and clinical function. These environmental conditions in the middle ear lead to postoperative retraction and adhesion of the newly‐formed tympanic membrane. Therefore, if the mucosa on the exposed middle ear bone surface can be rapidly regenerated after surgery, the surgical treatments for cholesteatoma and adhesive middle ear disease can potentially be improved. In this study, we successfully generated a cell sheet designed for the postoperative treatment of cholesteatoma. We used nasal cells to create an artificial middle ear mucosal cell sheet with a three‐dimensional (3D) configuration similar to that of the middle ear mucosa. The sheets consisted of multi‐layered mucosal epithelia and lower connective tissue and were similar to normal middle ear mucosa. This result indicates that tissue‐engineered mucosal cell sheets would be useful to minimize complications after surgical operations in the middle ear and future clinical applications are expected. Copyright © 2015 John Wiley & Sons, Ltd.