Near infrared spectroscopy of urine proves useful for estimating ovulation in giant panda (Ailuropoda melanoleuca)
Near infrared spectroscopy of urine proves useful for estimating ovulation in giant panda (Ailuropoda melanoleuca)
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DOI：
10.1039/c0ay00333f
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发表时间：
2010-11-01
影响因子：
3.1
通讯作者：
Tsenkova, Roumiana
中科院分区：
文献类型：
--
作者：
Kinoshita, Kodzue;Morita, Hiroyuki;Tsenkova, Roumiana
为了确定圈养雌性大熊猫的最佳繁殖时间，研究了近红外光谱（NIRS）技术在监测尿雌激素浓度中的应用。于2007年3月1日至25日采集了雌性大熊猫（Ailuropodamelanoleuca）53只的尿液样品。采用酶免疫分析法（EIA）测定雌酮-3-葡萄糖醛酸苷（E（1）G）浓度。在1100 ~ 2432 nm波长范围内获得了所有尿样的透射光谱采用偏最小二乘回归分析方法对样品进行分析，结果表明，EIA法测定的E（1）G浓度与NIR法预测的E（1）G浓度具有良好的相关性（R-2 = 0.94，SECV = 10.04 ng ml（-1））。类类比软独立模型（SIMCA）和移动主成分分析（MPCA）均能反映EIA测定的E（1）G浓度的时间变化（E（1）G浓度与SIMCA类间距离和MPCA指数的Pearson相关系数分别为r = 0.64和r = 0.81，P < 0.01）。至于MPCA指数，该指数在3月24日急剧下降，对应于指示排卵的E（1）G浓度的降低。最后，连续3天进行人工授精，包括高峰日3月24日，雌性妊娠。这些结果表明，NIRS和随后对相应尿液光谱数据的MPCA分析可以检测到纳克水平的动情周期期间尿液激素的变化。近红外光谱技术可以比EIA更快、更容易地找到繁殖的最佳时机，因此该技术可以用于该濒危物种的圈养繁殖。
The usefulness of near infrared spectroscopy (NIRS) to monitor urine estrogen concentrations was studied in order to determine optimal timing for breeding captive female giant pandas. NIR spectra of daily urine samples from a female giant panda (Ailuropoda melanoleuca) were acquired in the period between March 1st and 25th, 2007 (n = 53). Estrone-3-glucuronide (E(1)G) concentrations in the samples were also measured by enzyme immunoassay (EIA). Transmittance spectra of all urine samples were obtained in the wavelength range from 1100 to 2432 nm (excluding the range from 1884 to 2012 nm) with sample thickness of 1 mm. Partial least square regression was applied to the spectra and good correlation was obtained between E(1)G concentration measured by EIA and predicted values by NIR (R-2 = 0.94, SECV = 10.04 ng ml(-1)). The results of both soft-independent modeling of class analogy (SIMCA) and moving principal component analysis (MPCA) could detect the time changes in E(1)G concentration as measured by EIA (the Pearson's correlation coefficients between E(1)G concentration and the interclass distances of SIMCA or the index of MPCA were r = 0.64 and r = 0.81 respectively, P < 0.01). As for MPCA index, the index sharply dropped on March 24th corresponding to the decrease of the E(1)G concentration indicating ovulation. Finally, artificial insemination was performed for 3 consecutive days including the peak day, March 24th, and the female became pregnant. These results indicated that NIRS and the following MPCA analysis of the respective urine spectral data could detect the changes of urinary hormones during estrous cycle at a nanogram level. The NIRS can find the optimal timing for breeding quicker and easier than EIA, so this technique can be useful for captive breeding of this threatened species.