Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters
Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters
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DOI：
10.1016/j.sbi.2019.02.008
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发表时间：
2019-08-01
影响因子：
6.8
通讯作者：
Scheuring, Simon
中科院分区：
文献类型：
--
作者：
Heath, George R.;Scheuring, Simon
高速原子力显微镜（HS-AFM）的最新进展使得研究单个未标记跨膜通道和转运蛋白的构象动力学成为可能。改善环境控制与集成的非干扰缓冲液交换系统，这反过来又允许在HS-AFM操作过程中的条件的逐渐变化，提供了一个突破的结构滴定实验的性能。使用线扫描和高度光谱技术在时间分辨率方面的进一步进步表明，高速原子力显微镜可以测量毫秒到微秒的动态，使这种方法超越了当前不太直接的技术所提供的空间和时间限制。
Recent advances in high-speed atomic force microscopy (HS-AFM) have made it possible to study the conformational dynamics of single unlabeled transmembrane channels and transporters. Improving environmental control with the integration of a non-disturbing buffer exchange system, which in turn allows the gradual change of conditions during HS-AFM operation, has provided a breakthrough toward the performance of structural titration experiments. Further advancements in temporal resolution with the use of line scanning and height spectroscopy techniques show how high-speed atomic force microscopy can measure millisecond to microsecond dynamics, pushing this method beyond current spatial and temporal limits offered by less direct techniques.