Quantification of 2D elemental distribution maps of intermediate-thick biological sections by low energy synchrotron μ-X-ray fluorescence spectrometry
Quantification of 2D elemental distribution maps of intermediate-thick biological sections by low energy synchrotron μ-X-ray fluorescence spectrometry
复制标题
DOI：
10.1088/1748-0221/13/05/c05014
复制
发表时间：
2018-05-01
影响因子：
1.3
通讯作者：
Vogel-Mikus, K.
中科院分区：
文献类型：
--
作者：
Kump, P.;Vogel-Mikus, K.
同步辐射低能μ-X射线荧光光谱法定量中厚生物样品二维元素分布图的两种基本参数模型（SR-mu-XRF）被提出并应用于在两个低能量X射线荧光束线处用单色聚焦光子束激发的实验中的元素分析-TwinMic，Elettra Sincrotrone Trieste，意大利，和ID 21，ESRF，Grenoble，法国该模型假设中厚的生物样品组成的测量元素，可测量的光谱线的来源，并通过残留矩阵，它通过吸收影响测量的强度。在第一个模型中，假设样品的固定残差矩阵，而在第二个模型中，残差矩阵通过元素浓度的迭代细化和调整后的残差矩阵获得。从光电二极管或CCD相机的输出确定的生物样品中在每个扫描像素位置处的入射聚焦光束的吸收被应用作为量化的迭代过程中的控制。
Two fundamental-parameter (FP) based models for quantification of 2D elemental distribution maps of intermediate-thick biological samples by synchrotron low energy mu-X-ray fluorescence spectrometry (SR-mu-XRF) are presented and applied to the elemental analysis in experiments with monochromatic focused photon beam excitation at two low energy X-ray fluorescence beam-lines-TwinMic, Elettra Sincrotrone Trieste, Italy, and ID21, ESRF, Grenoble, France. The models assume intermediate-thick biological samples composed of measured elements, the sources of the measurable spectral lines, and by the residual matrix, which affects the measured intensities through absorption. In the first model a fixed residual matrix of the sample is assumed, while in the second model the residual matrix is obtained by the iteration refinement of elemental concentrations and an adjusted residual matrix. The absorption of the incident focused beam in the biological sample at each scanned pixel position, determined from the output of a photodiode or a CCD camera, is applied as a control in the iteration procedure of quantification.